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Q: How does Na-OH stop enzyme hydrolysis of polysaccharides?
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What is the purpose of adding NaOH to the mixture before taking the polarimeter reading?

It is to neutralize the solution and to stop the hydrolysis.


Why NaOH is used as stop solution to determine specific activity of alkaline phosphatase?

This serves two functions; first, it stops the enzyme-catalyzed reaction by changing the pH to one unsuitable for the enzyme (the enzyme is denatured at high pH and so unable to function). Second, it deprotonates the p-nitrophenol to give the yellow colored p-nitrophenylate.


What substance can be used to stop enzyme reaction?

prohibitors are used to enzyme reaction


Why you use ice in the hydrolysis of ethyl acetate?

to freeze the reaction mixture, means to stop the further hydrolysis of ethyl acetate so that our readings couldn't interrupt.


Does freezing a enzyme affect it?

When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.


What are the effects of freezing on enzyme activity?

When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.


Why would boiling an enzyme stop it from functioning?

Destroying the active site of an enzyme would no longer allow a substrate to bind to it, therefore stopping the enzyme from working.


Why do yeast don't have enzyme?

stop speaking jiberish dumbo


Explain the effects of pH on enzyme reaction rates?

A change in pH can denature an enzyme, meaning the reaction would stop.


What would happen to cellular respiration process if the enzyme for one step of the process for missing or defective?

If an enzyme in a sequence of enzyme-controlled reactions is missing or defective then the process will stop at that point. So respiration could proceed until it reached the reaction which needed the missing or defective enzyme at which point it would stop.


What would happen to cellular respiration process if the enzyme for one step of the process were missing or defective?

If an enzyme in a sequence of enzyme-controlled reactions is missing or defective then the process will stop at that point. So respiration could proceed until it reached the reaction which needed the missing or defective enzyme at which point it would stop.


What is the function of sulphuric acid in the experiment of enzyme activity?

H2SO4 is used to denature the enzyme and stop the reaction instantly. by adding H2SO4,it will prevent further reaction of the enzyme onto the substrate and the rate of enzyme reaction can be measured in the specific time