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How would the pH of the stain methylene blue affect the staining of bacteria?
Wiki User
∙ 12y ago
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
Wiki User
∙ 12y ago
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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Chemical used to make a specimen visible?
A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil
What is Eosin Dye?
It is a dye found in Eosin-methylene blue (EMB) agar, which permits the differentiation between enteric lactose fermenters and nonfermenters. The dye methylene blue inhibits the growth of gram positive bacteria. While the eosin acts as a pH indicator, measuring the levels of acid production due to the fermentation of lactose.Eosin is a stain that is used for staining the cells to observe the structurea nd number of cells. It is an acidophilic stain.
Why acid alcohol is used instead of ethyl alcohol in acid fast staining?
Acid alcohol destains non-acid fast bacteria but not Mycobacteria, which are resistant to the procedure due to the presence of mycolic acid. In the Ziehl Neelsen procedure, Mycobacteria remain red from the carbolfuchsin primary stain after destaining and non-acid fast bacteria (or tissue) which lose the primary stain during the destaining procedure are counterstained blue by methylene blue.
What is the practical importance in a medical lab of the acid-fast stain?
By this technique, we can diffentiate the acid fast and non acid- fast bacteria. The non acid-fast bacteria are M.tuberculosis and N.asteriodes. They are the causative agents for tuberculosis and nocardiosis respectively. The acid fast staining or the Ziel- Nielsen's staining is the only procedure to find out the above mentioned pathogens.
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Can Methylene blue used to stain bacteria?
Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.
What is secondary stain?
A secondary stain is Methylene blue. This type of stain is used in a acid fast staining. This type of staining test can determine medical conditions such as tuberculosis.
What color is simple staining on a bacillus and coccus using methylene blue stain?
Methylene blue stains everything blue.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
What is the standard stain for biological tissues?
Eosin and methylene blue are used for staining biolgical tissues.
What is the stain most useful in clinicians to decide for bacterial infection?
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
Why safranine and not methylene blue is used to stain plant cells?
Both are used in staining but for different purposes .
Chemical used to make a specimen visible?
A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil
What is the stain blue used to stain animal cells?
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
What kind of stain use in histology?
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
