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Q: How would you record your observation of a plate containing 305 colonies?
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What one plate would you first inspect to conclude that the transformation occurred successfully Why?

If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.


How do you determine the number of organisms in a water sample if your dilution plate has presence of spreading colonies?

To determine the number of organisms in a water sample choose a plate that has less than 300 but more than 30 colonies. Then count the number of colonies and record the results.


How you differentiate between Transformed bacterial colonies and satellite colonies?

Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.


What explains the observation that when a continental and oceanic plate collide the oceanic plate dives down under the continental plate?

The oceanic plate must be more dense than the continental plate for this to happen.


What is the continental plate containing Europe and Asia?

eurasian


How do colonies on the surface of a pour plate differ from those suspended in agar?

How do colonies on the surface of a pour plate differ from those suspended in the agar?


What is an assiette?

An assiette is a plate of food, a small plate containing the same food item prepared in different ways.


Why restreak isolated colony?

The isolated colonies for the new plate can be used to compare with the isolated colonies on the old plate. If the morphologies are the same, it can be assumed that pure culture is obtained.


What is a disk containing cell wall material called?

Cell Plate


What is considered a high plate count?

Usually hundreds of colonies (>300) is considered as a high plate count.


Is there a way to tell by casual observation if a car comes from a particular state?

check the license plate??


What if your instructor asks you to determine the number of organisms in a water sample observation of your dilution plates reveals?

I think you would eliminate plate counts that are not between 30-300 colonies. <30, because its too few to count, >300 too numerous to count.