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Aside from motility what facts learned from hanging drop preparation?
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
Is it always necessary to use a coverslip with a wet mount to prevent soiling the objective lens?
true
Is it necessary to add and drop water between the slide and cover slip?
For wet mount slides, yes.
Why would a wet mount be used?
The benefit of a wet mount is that you can view the specimen in its natural environment without killing it. If you do a wet mount you can get proper air around the microbes to view them for a while before they possibly die. If you do a hanging wet mount you have the capability to see them moving in an aerated manner.
Which works best with phase contrast microscopy wet mount or hang drop?
wet mount slide
What is the value of a wet mount preparation?
Wet mount preparation allows viewing of live and motile microorganisms which would be otherwise killed due to harsh staining procedures
What info can you observe on a wet mount that cannot be seen on a stained preparation?
The movement of a bacteria can be seen on a wet mount. This movement can not be seen in a stained preparation.
Why a smear preparation is preferred compare to a wet mount?
bcoz a heat-fix smear have a good contrast than the wet mount
What specimen preparation is best for viewing cell motility?
hanging drop
Aside from motility what facts learned from hanging drop preparation?
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
What Advantages of staining cells?
you could dry out the cell under the light, which will make them no longer viable. So if you want to study living cells, wet mounts are necessary.
Is it always necessary to use a coverslip with a wet mount to prevent soiling the objective lens?
true
Is it necessary to add and drop water between the slide and cover slip?
For wet mount slides, yes.
What advantages does hanging drop preparation have over a wet mount preparation?
Hanging drop slide is more resistant to evaporation because of the concave depression of the slide placed over the cells along with the petroleum jelly prevents drying. Wet mounts can dry out fairly quickly.
What is the difference between wet mount and dry mount?
- a wet mount is freshly prepared while a dry mount is pre-prepared and sealed. - the specimen is alive in a wet mount while in a dry mount the specimen is dead - air bubbles can be trapped in a wet mount but not in a dry mount - wet mounts are priceless while dry mounts costs
Why would a wet mount be used?
The benefit of a wet mount is that you can view the specimen in its natural environment without killing it. If you do a wet mount you can get proper air around the microbes to view them for a while before they possibly die. If you do a hanging wet mount you have the capability to see them moving in an aerated manner.
What is to mount a slide?
A wet mount slide is when a solution is applied to a microscope slide with whatever subject is being studied, typically under a slip cover.
