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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
Wandawebb
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Wiki User
∙ 15y ago
Wiki User
∙ 13y agoActually methylene blue can be prepared as both a basic stain or an acidic stain.
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Which of the following is an example of an acidic stain?
Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red
Why pka for ethylacetate is 23 whereas that for ethyl acetoacetate is 11?
The most acidic proton in ethylacetoacetate is the methylene between the two carbonyl groups. The anion resulting from deprotonation of this methylene can be resonance stabilised onto both carbonyls, making it more stable and therefore easier to form. The net effect is the proton is more acidic compared to ethylacetate where only one resonance structure can be drawn.
What is reactive methylene compounds?
The hydrogen attached to a saturated carbon atom containing strong electron withdrawing groups in acidic form reacts with base to generate carbanion .such compounds which contain acidic CH2 group are called active methylene compounds . eg:CH3-CO-CH2-COO-C2H5 (ethyl aceto acetate
Is crystal violet a basic or an acidic dye?
Crystal violet, also called methyl violet 10B, is a dark purple staining dye used in biology and microbiology which, like many staining dyes, is acidic.The acidic nature of crystal violet dye has many implications in chemistry, determining how it will interact with other substances, which microorganisms will absorb or repel it, and how it will effect structures such as cell walls or DNA.Since many acidic staining dyes are caustic or carcinogenic, they can pose logistical, legal, and ethical problems during disposal. Numerous studies have been launched in order to find cost-effective ways to degrade, absorb, neutralize, or deactivate these chemicals.Knowlege of the acidic nature of such substances is among several important considerations in devising such approaches.
How do antiseptics kill bacteria?
antiseptics target the bacteria and alter the PHof their enviroment toa more basic or acidic level in which they cant live or grow in
Why methylene blue inhibits gram positive bacteria?
because it is acidic?
Why an acidic stain is used in negative staining?
Acidic dyes are negatively-charged dyes. Since bacteria are also negatively-charged, they will repel the acidic dyes. So, instead of staining the bacterium itself, it will be the background that will be colorized....
Staining preparation with eosin and methylene blue?
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
Is a nucleus acidic or alkaline in a staining of cheek cells fro microscopic analysis?
the nucleus is acidic if you take methylene blue and stain an animal cell (ex: cheek cell from your own body) it stains deep blue. this shows the prescence of an acid
What causes a stain to adhere to bacteria cells and why all colored dyes not necessarily useful for simple staining?
The causes a stain adheres to bacterial cells is the color-bearing ions (chromophores) and they might be positively charged (cationic), such as methylene blue, basic fuchsin, and crystal violet, because bacteria are negatively charged and anionic chromophores, such as eosin, will not stain bacteria because of the electrostatic repelling forces that are involved.
What Name three basic stains?
Bacterial staining: These are the methods for staining various parts of bacterial cell.1. Gram staining: Used for primary identification of two major groups of bacteria - Gram Positive and Gram Negative.2. Cell wall staining: shows cell wall of bacteria.3. Nuclear staining: Though bacteria do not possess true Nucleus, an area high concentration of Nucleic acids can be stained.4. Lipid stain5. Spore stain6. Flagella7. Capsule8.Meta chrimatic granules.9.. Spirocheate staining: this method is used to show presence of spirocheates in a smear.
Which of the following is an example of an acidic stain?
Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red
What bacteria contributes to the acidic condition of the skin?
propionicbacterium
Does pickling food kill bacteria?
It creates an acidic environment which many bacteria cannot tolerate.
Why pka for ethylacetate is 23 whereas that for ethyl acetoacetate is 11?
The most acidic proton in ethylacetoacetate is the methylene between the two carbonyl groups. The anion resulting from deprotonation of this methylene can be resonance stabilised onto both carbonyls, making it more stable and therefore easier to form. The net effect is the proton is more acidic compared to ethylacetate where only one resonance structure can be drawn.
What is reactive methylene compounds?
The hydrogen attached to a saturated carbon atom containing strong electron withdrawing groups in acidic form reacts with base to generate carbanion .such compounds which contain acidic CH2 group are called active methylene compounds . eg:CH3-CO-CH2-COO-C2H5 (ethyl aceto acetate
