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Methods The determination of febuxostat and the separation of its related substances was performed on a C18 column( 200 mm ×4.6 mm, 5 μm ) . The mobile phase was methanol-acetonitrile-0.05%(w)phosphoric acid (V:V:V=24:46:30) . The flow rate was 1.0 mL·min-1. Ultraviolet absorption detector was set at 315 nm and column temperature at 35 ℃. Results The linear range of febuxostat was between15.7 and 94.3 mg·L-1 ( r = 0. 999 8) . The average recovery was 100.5% with RSD of 1.0%.The related substances of febuxostat could be completely separated from febuxostat. The limit of detection (LOD) was 0.5 ng.
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What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Asenapine maleate method of analysis by HPLC?
yes
What are the guidelines for method development of hplc according to ich?
the same guidelines for method validation
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What is assay by HPLC?
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
How buffer concentration effects on retention time in hplc method?
concentration of buffer effects in buffer strength
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
How you find out Pka value of organic compound for HPLC method develoment?
If you can't find it in the literature it can be determined experimentally by titration.
Why the pH of buffer is not changes in robustness of validation of hplc method?
it must change by (+- 0.3) to have control in pH meter error
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
