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Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
How to choose using an MTT assay vs Crystal Violet Staining?
the same
What is positive staining?
cells which take up crystal violet stain and retain them because of smal pore size
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
How to choose using an MTT assay vs Crystal Violet Staining?
the same
Why you should shake the crystal violet bottle before staining?
we must shake it because crystals of crystal violt preceptiate in the bottle
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
What is positive staining?
cells which take up crystal violet stain and retain them because of smal pore size
What is the principle of crystal violet staining in monolayer culture?
Crystal Violet is a dye that accumulates in the cell nucleus. The fixed dye, measured photometrically after solubilization, correlates with the nuclear DNA content and thus with cell number.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why use citric acid in crystal violet staining?
Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid. As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately. Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
