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Yes. EMBA is a differential media for E.coli. E.coli will grow as dark blue/ purple colonies with green metallic sheen. Sometimes the green metallic sheen is more difficult to identify if you only are allotted a certain time to check results. Sometimes the presence of the green metallic sheen is for a fleeting time. Evidence of the green metallic sheen will be a glossy sheen to the inoculation streak. diana

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15y ago
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13y ago

metallic green

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Q: What color would E. coli appear if they grow on eosin methylene blue?
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Staining preparation with eosin and methylene blue?

Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.


Will staph grow on EMB agar?

No, it will not. Methylene blue agar is used to identify gram negative bacteria, staphylococcus is gram positive.


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How would the pH of the stain methylene blue affect the staining of bacteria?

the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.


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