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The main substrate for for Taq polymerase is magnesium chloride
Wiki User
∙ 14y ago
Wiki User
∙ 11y agodeoxyribonucleoside triphosphate (dNTPs).
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What is synthesized by RNA polymerase?
Polymerases are the enzymes that replicate and build nucleic acids. DNA polymerases synthesize DNA, RNA polymerases synthesize RNA. Purified polymerases are essential to carrying out the PCR reaction.
What enzyme reads the DNA template?
Various DNA polymerases read the DNA template during replication of DNA. Various RNA polymerases read the DNA template during transcription.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases
What are the two major functions that DNA polymerase performs?
DNA Polymerases are responsible for the addition of nucleotides to the new strand of DNA, and for replacing the RNA primers.
How are the accuracy of DNA and RNA assured?
Most DNA Polymerases and RNA polymerases have what is known as "proof-reading activity". This is the ability of the enzymes to check what bases they have added during DNA replication (in the case of DNA Polymerase) or transcription (in the case of RNA Polymerase), and if an error is found, splice it out and replace it with the correct base. The mode of action depends on the enzyme in question - some use endonucleases, and some use exonucleases; some work 5'-3' while others work 3'-5'. Also note that I said MOST polymerases have proof-reading capabilities...there are a few which do not (or don't proof-read very well).
What are the Examples of intra cellular enzymes?
RNA/DNA polymerases - Reverse Transcriptase. Helicase. Binding proteins. Enzymes simply act on a substrate - there are tonnes.
What is enzyme that adds nucleotides to exposed nitrogen bases?
DNA Polymerases DNA Polymerases
What is synthesized by RNA polymerase?
Polymerases are the enzymes that replicate and build nucleic acids. DNA polymerases synthesize DNA, RNA polymerases synthesize RNA. Purified polymerases are essential to carrying out the PCR reaction.
What enzyme reads the DNA template?
Various DNA polymerases read the DNA template during replication of DNA. Various RNA polymerases read the DNA template during transcription.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases
What are the two major functions that DNA polymerase performs?
DNA Polymerases are responsible for the addition of nucleotides to the new strand of DNA, and for replacing the RNA primers.
What do you need to copy DNA?
Dna polymerases occur in a wide variety of Descriptions.
What are the enzymes that are responisble for adding nucleotides to the exposed DNA bases during replication?
DNA polymerases. As an incoming nucleotide base-pairs with it's complement on the template strand, a DNA polymerase adds it to the end of the growing daughter strand (polymer).
What enzyme is used to rebuild DNA strands?
My guess is that you're thinking of DNA polymerase.
What enzyme is responsible for catalyzing the formation of the two new DNA strands?
DNA polymerases
What catalyzes the linking together of nucleotide subunits?
DNA Polymerases
What is responsible for building the DNA strand?
DNA is build from another template DNA molecule using DNA polymerase, among other enzymes.
