octopine
agrobacterium tumefaciens
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
transfer of gene with the help of Ti plasmid present in Agrobacterium which has T-dna region by which gene van transfer.
Agrobacterium tumefaciens
Ti plasmid functions to induce turmor or a desease known as "crown gall" to the most dicot (rarely monocot) plants. Transfer DNA or T-DNA will be released during the infection process into the plant cell and integrate with the DNA host. Hence, the plant host is already infected. That's the important function of the Ti plasmid, if there are no such plasmid exist, then the agrobacterium lost its pathogenic function.
agrobacterium tumefaciens
ends of plasmid of agrobacterium.
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
transfer of gene with the help of Ti plasmid present in Agrobacterium which has T-dna region by which gene van transfer.
Agrobacterium tumefaciens
Ti plasmid functions to induce turmor or a desease known as "crown gall" to the most dicot (rarely monocot) plants. Transfer DNA or T-DNA will be released during the infection process into the plant cell and integrate with the DNA host. Hence, the plant host is already infected. That's the important function of the Ti plasmid, if there are no such plasmid exist, then the agrobacterium lost its pathogenic function.
Using agrobacterium is the most common way of genetically modifying plants. Inside the bacterium there is a circular piece of DNA called a plasmid, which also has a chromosome. This TI plasmid is tumor inducing but it transfers the plasmid DNA by infecting the plants DNA (it cuts the chromosome of the plant and inserts the plasmid). The bacterium live inside the tumor of the plant. Scientists tooks the T dna, removed the tumor causing genes, and left the DNA splicing genes. They then use the bacterium to transfer the DNA they want.
Agrobacterium-mediated transformation using a plasmid containing a gene for GFP
recombinant dna
1)Allow transfer of genes from bacterium to plant by the use of plasmid, known as Ti plasmid. 2) Allow to be used as a tool for plant breeding e.g. any desired gene, such as insecticidal toxin genes or herbicide resistant genes, can be engineered into the bacterial DNA then transferred into plant genome 3) Agrobacterium shortens the conventional plant breeding process 4) Allows entirely new (non-plant) genes to be engineered into crops 5) Is a highly effective biological control system for disease (e.g. crown gall disease). 6)Used for plant improvement (2 above)
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
what is the function of the plasmid