it is most likely an algae that has been flocked to the bottom by shocking.your chemicals have done there job now it is time to vacuum up the dead algae so it does not start to grow again much like a weed. If you keep your Alkalinity,PH and Chlorine stable you will not have to ad other chemicls with the exception of non-chlorine shock.Remember every extra chemical you add to your pool or spa is providing food for algae. kenny Kummer brody Chemical
E. coli is a gram negative bacteria, meaning that it has a cytoplasmic lipid membrane, a peptidoglycan layer, and a (LPS) lipopolysaccharide layer. As a result, e. coli stains a pink colour on a gram stain from the counterstain saffranin. Gram positives stain purple retain the crystal violet dye even after washed with a decolouring solution.
The first stain is crystal violet, next iodine is added which acts as a mordent. Then, alcohol is added which will wash away the purple color from any gram negative bacteria. The purple color will stay in the gram positive bacteria due to their thich peptidoglycan layer. Lastly, Safranin is added as a counter stain. Gram - is red Gram + is purple
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
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