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thermostable enzyme is kind of enzyme that could keep working (do their function) through heating condition. Some of enzyme are unstable through heating and get denaturation ( loss of 3D protein shape) in 40-60 degree Celsius. Example: kind of alfa amylase enzyme that stable in 60 degree C.
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Is vent polymerase a thermostable enzyme?
Yes. Vent polymerase is thermostable and is commonly used for PCR
Moderately thermostable glassware?
Thermostable glass is a borosilicate glass.
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What is the significance of thermostable polymerase in DNA technology?
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
What is the Difference between enzyme and regulatory enzyme?
An enzyme is one kind of protein that can catalyze a specific reaction whereas a regulatory enzyme is the enzyme which can regulate a series of reaction which undergo in the living organism. So we can say every enzyme is not a regulatory one but the regulatory enzymes are obviously a special kind of enzyme.
Is vent polymerase a thermostable enzyme?
Yes. Vent polymerase is thermostable and is commonly used for PCR
Moderately thermostable glassware?
Thermostable glass is a borosilicate glass.
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What has the author CM Menendez written?
C.M Menendez has written: 'Producation of the thermostable dna polymerbase from thermus aquaticus in escherichia coli'
What has the author Alexander Fainleib written?
Alexander Fainleib has written: 'Thermostable polycyanurates' -- subject(s): Polyurethanes, Triazenes, Fire resistant polymers
What is the significance of thermostable polymerase in DNA technology?
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What to enzymes do in an enzyme-substrate?
in an enzyme-substrate complex, the enzyme acts on the substrate .
Does enzyme has proteins?
An enzyme is a protein
Who discovered taq polymerase enzyme?
Alice Chien, et al. were the first to describe the Taqpolymerase in 1976.Then, Saiki et al. were the first to employ the polymerase in a published study.Chien A, Edgar DB, Trela JM. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 1976;127:1550-1557.Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-491.
