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2-Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract.
It may also help to denature proteins by breaking disulphide bonds between cysteine residues.
Wiki User
∙ 14y ago
Wiki User
∙ 14y agoEthanol is a dehydrating agent. In DNA it is used for the precipitation of DNA molecule. When a molecule is to be precipitated it should be dehydrated as the water molecules forming a film around it prevent their interaction. In DNA isolation the DNA molecules are dehydrated by ethanol.
Wiki User
∙ 12y agoB Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract.
It may also help to denature proteins by breaking disulphide bonds between cysteine residues.
Wiki User
∙ 10y agoEDTA is a chelating agent. It chelates out divalent cations (mainly Mg2+), essential for the action of nucleases, from the solution. Thus it prevents degradation of DNA by DNase.
Wiki User
∙ 15y agorole
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Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
