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SDS PAGE electrophoresis is an important method in the separation of proteins. it can be use to identify and isolate proteins aswell as determine if a protein solution is pure or contaminated

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15y ago
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12y ago

SDS allows proteins to be separated on the basis of approximate mass.

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Q: What is the advantage of adding SDS to gel electrophoresis?
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Related questions

What is vertical gel electrophoresis unit?

Samole moves from top to bottom is called vertical gel system, for example a SDS PAGE gel.


DNA fragments can be separated and analyzed by?

Pulse field gel electrophoresis is used to separate DNA fragments by their size.


What is used to sort DNA into different lenghts?

Gel Electrophoresis


what is the gel in Gel Electrophoresis?

For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis


Can a gel electrophoresis be performed both horizonnally and vertically?

yes for example 2D gel electrophoresis


A technique that can be used to compare the DNA of two or more plants is?

Gel electrophoresis


What is a common name for gel electrophoresis?

gel


Where can one learn more on gel electrophoresis?

To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.


What has the author A J Houtsmuller written?

A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins


How does time influence the rate of electrophoresis?

Hi, I assume you mean gel electrophoresis of proteins (commonly done in a polyacrylamide gel e.g. SDS-PAGE) or agarose gel electrophoresis of DNA. Generally, as electrophoresis is allowed to proceed for a long time, the gel and the buffer in which it is submerged in becomes heated (due to Joule heating effects of the current supply). The heating causes the pores in the gel matrix to lose their definition (due to flaccidness induced upon the polyacrylamide / agarose matrix strands within the gel) and the sample molecules (being electrophoresed) can now easily 'force' their way through the meshwork of fibres within the gel, thus creating an illusionary aspect of 'enhanced rate of migration' (i.e. 'increased rate of electrophoresis'). Hope this answers your query. Thanks and Regards, Shiraz


Process that restriction fragments of DNA are separated from each other by the use of electricity?

The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field


Can gel electrophoresis be used to determine the purity of an enzyme?

Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.