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What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What are temperate phages?
A phage that can enter into lysogeny with its host. A phage that can become a prophage.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
The part that remains outside is called the capsid or protein coat.
How do restriction enzymes create recombinant DNA?
There are many methods, though one of the most common is the use of restriction endonucleases. These enzymes can be used to cut DNA fragments at specific locations. Cut DNA fragments will recombine into new orders, which are sealed using DNA ligase. A selection process must be used to locate the desired recombinant DNA, since it will be in a mixture of various undesired recombinations.
What is the difference between a virulent phage and a temperate phage?
In virology, temperate refers to the life cycle some phages are able to perform. Atemperate phage can integrate its genome into its host bacterium's chromosome, becoming a lysogen known as a prophage. A temperate phage is also able to undergo lytic life cycles, where the prophage is expressed, replicates the phage genome and produces phage progeny and the progeny phage leave the bacterium. The virulent phages have only lytic lifecycles and thus infection results in the host cell's death (due to lytic cell destruction-the phage replicates itself and then bursts the cell, releasing many copies).
Lamda phage of E. coli?
It is the 'T4phage' or 'coliphage lambda'.
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What are two types of vector used in recombinant DNA technology?
there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system the other cloning vectors are m13 which is the oldest one. and after the above all are:- BAC(bacterial artificial chromosome) YAC(yeat artificial chromosome) TAC(transformation-competent artificial chromosome)
What is the insert capacity of a P1 phage in DNA cloning?
You can add maximum 70-100 kb of genetic material in a P1 phage vector.
What does a phage inject when it attaches to a bacterium?
Dna and phage body
What is the need for phage titration?
PHAGE TRTRATION IS DONE SO AS TO FIND THE AMOUNT OF PHAGE PARTICLES PRESENT IN THE STOCK PHAGE TRTRATION IS DONE SO AS TO FIND THE AMOUNT OF PHAGE PARTICLES PRESENT IN THE STOCK
What is another term for a lysogenic phage?
temperate phage
A virus that reproduces in a bacterium is called?
phage
When phage attaches to a bacteria the phage injects what?
DNA single
What is temperate phage superinfection immunity?
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
What are temperate phages?
A phage that can enter into lysogeny with its host. A phage that can become a prophage.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
The part that remains outside is called the capsid or protein coat.
