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What is the colonial morphology of clostridium tetani in thioglycollate medium?
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How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
G+ and tan
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
G+ and tan
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
What is the colony morphology of Edwardsiella tarda?
On a TSA plate colonies are of medium size, irregular, raised, translucent, and smell of rotten eggs.
What growth requirements would you need in order to grow Clostridium perfringens microbe in a lab?
clostridium perfringes grow in blood agar/robertson's cooked meat medium/thiglycollate broth.serum or egg yolk agar are used for identification test i.e. naglers test.
What is the chemical composition of fluid thioglycollate medium?
Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride 2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g Final pH: 7.1 ± 0.2 at 25°C Formula may be adjusted and/or supplemented as required to meet performance specifications
