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How does ethidium-bromide stain DNA?
Ethidium bromide binds with DNA and slips in between its hydrophobic base pairs and stretches the DNA fragment, removing water molecules from the ethidium cation. The result of this dehydrogenation is an increase in fluorescence of the ethidium (as well as the cell).
What does ethidium bromide stain in a cell?
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
What are the dangers of ethidium bromide?
Studies are inconclusive that ethidium bromide, an inhibitor for cell growth, is not shown to be toxic in humans, but the metabolites may exude mutagenic characteristics.
What is copulatory organ?
any organ that is used to effect insemination.
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What is the effect does the disease or malfunctin have on the function of the organ system in the lungs?
What is the effect does the desease or malfunctin have on the function of the organ system in the lungs?
What organ does tobacco effect?
the lungs.
What organ does Cirrhosis effect?
Liver
Why dont you use ethidium bromide staining for non denaturing PAGE gels?
Because PAGE gels are used to look a protein and ethidium bromide only stains double stranded nucleic acids.
Are gas containing organ injuries attributed to thermal effect?
Primary blast effect
The effect in organ transplant?
ones live can be saved
What organ system does cancer effect?
the tuhod mo
