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Many DNA polymerases from organisms are not suitable for PCR because they do not possess the optimal features required for the enzymatic reactions involved in PCR, such as high processivity, thermostability, and fidelity. PCR generally requires a DNA polymerase that can withstand the high temperatures used during the process without denaturing. Taq polymerase, isolated from the thermophilic bacterium Thermus aquaticus, is commonly used for PCR due to its ability to function at high temperatures.
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Is DNA polymerase used in the process of transcription?
No, DNA polymerase is not used in the process of transcription. Transcription is the process of making an RNA copy of a gene from DNA, and it is carried out by an enzyme called RNA polymerase. DNA polymerase is primarily involved in the process of DNA replication.
Enzyme used to position nucleotides during DNA replication?
DNA polymerase is the enzyme responsible for positioning nucleotides during DNA replication. DNA polymerase can add nucleotides to the growing DNA strand in the 5' to 3' direction and proofread for errors in base pairing.
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
What is a polymerase commonly used for?
A polymerase is commonly used for amplifying DNA in a process called PCR (polymerase chain reaction). PCR is used to make copies of specific DNA sequences, which is essential for various genetic testing, molecular biology research, and diagnostic applications.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
What organisms that have DNA as their genetic molecules have the enzymes DNA polymerase?
Humans, practically all organisms. The enzyme DNA polymerase is essential in DNA replica which is also a vital biological reaction. They are responsible for attracting free nucleotides and assorting them into the corresponding bases of the parental DNA.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
Is DNA polymerase used in the process of transcription?
No, DNA polymerase is not used in the process of transcription. Transcription is the process of making an RNA copy of a gene from DNA, and it is carried out by an enzyme called RNA polymerase. DNA polymerase is primarily involved in the process of DNA replication.
Enzyme used to position nucleotides during DNA replication?
DNA polymerase is the enzyme responsible for positioning nucleotides during DNA replication. DNA polymerase can add nucleotides to the growing DNA strand in the 5' to 3' direction and proofread for errors in base pairing.
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
What is a polymerase commonly used for?
A polymerase is commonly used for amplifying DNA in a process called PCR (polymerase chain reaction). PCR is used to make copies of specific DNA sequences, which is essential for various genetic testing, molecular biology research, and diagnostic applications.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What is main function of DNA polymerase?
The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
What enzyme is used to join DNA nucleotides?
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
