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Why didn't we inoculate MUG agar directly and bypass lactose broth?
Wiki User
∙ 13y ago
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
Wiki User
∙ 13y ago
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Why isn't a pH indicator needed in the lactose broth fermentation tubes?
not sure bro
What is an inoculating medium?
A media which is used to inoculate the bacterium for the culture is a inoculation media. Based on the bacteria, one can use LB media or nutrient broth or any other special media for this. The goal is to let the bacterium grow well from the inoculation culture or colony.
Explain the results in the sterilized and not-sterilized broth?
Sterilized broth is clear, meaning no bacterial growth. Non-sterilized broth is cloudy.
How long has broth been in our culture?
Broth has been in our culture since the beginning of time.
What is ratio between blood and broth in blood broth?
blood should be 5% by solution
How are acids detected in a culture medium?
use glucose phosphate broth , inoculate the organism in that broth and incubate at 37 degree celsius for 24 hrs.
Why isn't a pH indicator needed in the lactose broth fermentation tubes?
not sure bro
Composition of double strength lactose broth?
Double strength lactose broth typically contains peptone, yeast extract, lactose, and possibly other nutrients or supplements. It is commonly used for the cultivation of coliform bacteria, such as E. coli, in microbiology labs.
Why isn't a pH indicator needed in the lactose broth fermentation tube?
not sure bro
Saline lactose broth sterilization method?
Saline lactose broth can be sterilized by autoclaving at 121°C for 15 minutes. This process ensures the elimination of any microorganisms present in the broth, making it suitable for culturing bacteria under sterile conditions. It's essential to follow proper aseptic techniques when handling the sterilized broth to prevent contamination.
What reaction would you predict from an organism growing in litmus milk that has the following results in other media?
Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue
What is the composition of selenite f broth?
Selenite-F Broth Approximate Formula* Per Liter Purified Water Pancreatic Digest of Casein ....................................................5.0 g Lactose ......................................................................................4.0 g Sodium Selenite ........................................................................4.0 g Sodium Phosphate ..................................................................10.0 g *Adjusted and/or supplemented as required to meet performance criteria.
Preparation of penicillin by submerged culture method?
The penicillium is grown in a broth of corn steep liquor, lactose, and calciumcarbonate. Filter the broth through a qualitative paper filter to remove the pencillium. Add activated carbon to absorb the pencillin (allow carbon to soak for 15min). remove carbon via filter and place in acetate.
What is an inoculating medium?
A media which is used to inoculate the bacterium for the culture is a inoculation media. Based on the bacteria, one can use LB media or nutrient broth or any other special media for this. The goal is to let the bacterium grow well from the inoculation culture or colony.
Biochemical test for rhizobium colony?
The primary biochemical test for distinguishing Rhizobium colonies is the ability to fix atmospheric nitrogen, forming nodules on legume roots through a symbiotic relationship. Other tests can include nitrate reduction, utilization of sugars like sucrose or mannitol, and staining characteristics with specific dyes like carbol-fuchsin. These tests help identify Rhizobium strains and ensure their effectiveness in promoting plant growth.
Can chicken broth cause salmonella?
Yes, if the chicken used in the broth has salmonella, then it is possible that the broth will have it as well.
How do you use broth in a sentence?
i want chicken broth
