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What is meant by the term ''fixing the smear''?
Sumaira sultana
What is the purpose of fixing the smear?
Heat fixing a smear kills the bacteria with minimal distortion, allows for better staining, and firmly affixes the bacteria to the slide. Chemical fixing is used to preserve fine cellular structures and might stop internal processes in place, protect the cell from damage, or strengthen the cell's structure.
Why was koch's recommemdation for fixing and staining important for microbiology?
Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
Why acetone free methanol in lieshmans stain?
methanol for fixing the smear and acetone free to avoid cell membrane lysis
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
Is the endometrial biopsy necessary if you have already gone through menopause have a normal Pap Smear and are not experiencing any pain?
If you have bleeding after menopause, an endometrial biopsy is necessary.
What is the appropriate geometric fixing of two atoms which is necessary to start chemical rearrangement called?
fusion
Is presence of grease or dirt on a glass slide going to result in poor smear preparation?
The smear will not adhere well if there is grease on the slide.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
