The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
smear will be washed( no smear will be left on the slide)
It dries the smear and fixes the cells to the slide
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
smear will be washed( no smear will be left on the slide)
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
It dries the smear and fixes the cells to the slide
heat fixing your slide causes the cells to stick to the slide, without that the cells will be washed right off and there will be nothing left to observe.
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.