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What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
What is a stain used to make chromosomes visable called?
which stain is used to colour chromosomes
What is the stain used to staines the cell membrane?
we can use feulgen's method ,in which malachite green stain is used to stain the nucleus
What stain is used to stain cheek cells?
methelyn blue
The name given to a liquid used to make cells structures easier to see?
Stain . Iodine is one.
Is mordant used in acid fast stain?
The heat is the mordant for an acid-fast stain
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
Why is size more accurate in an acidic or negative stain than in a simple or basic stain?
Since there is no heat fixing or strong cemicals are used the bacteria are less distorted
Why is it necessary to heat the malachite green?
Malachite green is used as a primary stain in bacteria. It is necessary to heat the malachite green in order to penetrate the endospore of the bacteria.
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What kind of stain are used to stain plant and animal cells?
Methylene blue stain is used to stain plant and animal cells.
What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
What are the materials used in capsule stain?
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
What is a stain used to make chromosomes visable called?
which stain is used to colour chromosomes
