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because they cannot be differentiated from inclusions
of stored material without a special stain .
Wiki User
∙ 14y ago
Wiki User
∙ 13y agoThe heat helps the stain penetrate the endospore wall
Wiki User
∙ 13y agothe heat helps the stain penetrate the endospore wall.
Wiki User
∙ 12y agoSpore contain keratin protein that protects spore. Steam is necessary so that Malachite Green can enter the cell ad retain there.
Wiki User
∙ 12y agoThe heat helps the stain penetrate the endospore wall.
Wiki User
∙ 9y agoThe steaming helps the malachite green to permeate the low permeability spore wall.
Wiki User
∙ 15y agoHelps the dye permeate the spore wall.
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Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
What if You did not apply heat during the application of the primary stain?
if you didnt apply heat or Clorox, then the stain would not go away completely and we all don't want that!
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Are bacterial plasmids required for host growth and production?
7. Which of the following does NOT apply to plasmids?A. They are essential for the growth of the cell.B. They are composed of DNA.C. They multiply independently of the chromosome.D. They may pass from cell to cell in recombination
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
Can you mix processing lotion with henna and apply it to relaxed hair?
Yes, but you run the risk of permanently staining it unless your hair is perfectly relaxed. Try using some scented candles during the proccess.
Is it true that granite counter tops are prone to staining?
Granite countertops can be very appealing and high quality. They are easily damage and can be prone to staining. Yes it is true that granite countertops are more proned to staining. You can apply a sealant to your countertops to help prevent stains and damage.
How do you apply semitransparent deck stain?
Use a roller or staining pad to apply the oil or water-based semi-transparent stain. Go in the direction of the grain and do not allow the stain to puddle.
How do you enter the BNSF mechanical management trainee program?
apply during the fall apply during the fall
Can a photography student save on wireless camera's during tax free weekend?
Wireless cameras are in fact available at Best Buy. Unfortunately, because cameras are not considered an essential for school supplies, they do not apply for the tax-free status.
What happens to people inside a car during collision and laws that apply?
What happens to people inside a car during a collision and the laws that apply
Can I get a clean car with an at home fabric cleaner?
You can get an anti staining protector for your carpet. Just apply it to your seat and it should be good to go for several months. It will guard against coffee spills and stains.
When should you apply sealer to VCT?
When the VCT is old and worn. When there is possibility of staining. When there is high traffic. When you want the floor to really shine after applying floor finish over 2 coats of sealer.
How can I apply for money during my fifth year of college?
Where can I apply for help in my 5th year in college
How do you apply for engineer jobs?
You can apply for engineer jobs by applying to companies that require these kinds of skills. In order to be an engineer, it is essential almost always to also have a college degree and a strong background in engineering.
Can you apply Travocort cream in the viginal area during pregnancy?
No...
