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What is the reaction mechanism of orcinol in the estimation of RNA?
Wiki User
∙ 12y ago
Furfural cannot be formed from ribosides until the glycosidic
linkage has been split; the purine-ribose links of RNA are easily
hydrolysed by hot acid, while the pyrimidine-ribose links are much more
resistant, and the orcinol method is commonly supposed to determine only
the purine-bound ribose of RNA.
Wiki User
∙ 12y ago
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What does bials orcinol determine in nucleic acid?
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
Why is RNA interferon discovery important?
RNA carries out several important roles. There are 3 main types of RNA, messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). mRNA carries a copy of the instructions for creating proteins from the DNA in the nucleus to the ribosomes for translation. rRNA makes up part of the ribosomes. tRNA carries amino acids (the building blocks of proteins) to the ribosomes
What is synthesized by RNA polymerase?
Polymerases are the enzymes that replicate and build nucleic acids. DNA polymerases synthesize DNA, RNA polymerases synthesize RNA. Purified polymerases are essential to carrying out the PCR reaction.
How do you express The central dogma of biology or the mechanism of reading and expressing genes in all living things?
The "central dogma" was that the flow of information always went from DNA to RNA to protein. This assumption was discarded with the discovery of reverse transcriptase, which allowed information to move from RNA to DNA.
During DNA duplication primer called RNA primer why?
The initiation of the reaction is favoured energetically by formation of this RNA-DNA hybrid.This hybrid is more stronger than DNA-DNA hybrid
What is the theoretical result with orcinol reagent?
The initial yellow color of the Orcinol gets a green tinge upon heating with RNA.
What does bials orcinol determine in nucleic acid?
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
What is Principle behind using orcinol reagent in extraction of RNA from yeast?
The method depends on conversion of the pentose, ribose in the presence of hot acid to furfural which then reacts with orcinol to yield a green color. The color formed largely depends on the concentration of HCl, ferric chloride, orcinol, the time of heating at 100°C etc up to certain maxima.
What sugar does RNA have that is different from the sugar in the morning?
Which mechanism explains the phenomenon
Is RNA a thought to be the oldest and most primitive coding mechanism for life?
Yes it is the oldest and most primitive coding mechanism
How could RNA have become involved in the mechanism for protein translation?
natural selection favored RNA molecules that synthesized catalytic proteins
What is the indicator for nucleic acids?
Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl). This method requires the following reagents 1. Orcinol reagent : 6% orcinol in 95% ethanol. 2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.)) Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test. This method requires the following reagents one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization. Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution. This method requires the following reagents Hydrochloric acid, 1Normal Schiff's reagent (made from pararosanilin treated with sulphurous acid) Light green, 1% aqueous (can be replaced with Fast green FCF)
Antitermination of RNA synthesis is a major mechanism of regulation in?
lytic phase of lambda phage
What is the name of the chemical reaction when a strand of messenger RNA is formed from individual nucleotides?
Condensation!
What is the testing indicator for nucleic acids?
Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl). This method requires the following reagents 1. Orcinol reagent : 6% orcinol in 95% ethanol. 2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.)) Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test. This method requires the following reagents one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization. Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution. This method requires the following reagents Hydrochloric acid, 1Normal Schiff's reagent (made from pararosanilin treated with sulphurous acid) Light green, 1% aqueous (can be replaced with Fast green FCF)
Why is RNA interferon discovery important?
RNA carries out several important roles. There are 3 main types of RNA, messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). mRNA carries a copy of the instructions for creating proteins from the DNA in the nucleus to the ribosomes for translation. rRNA makes up part of the ribosomes. tRNA carries amino acids (the building blocks of proteins) to the ribosomes
What is synthesized by RNA polymerase?
Polymerases are the enzymes that replicate and build nucleic acids. DNA polymerases synthesize DNA, RNA polymerases synthesize RNA. Purified polymerases are essential to carrying out the PCR reaction.
