0
Methylene blue can be used to prepare slide for animal cells. For example, if you want to examine a piece of your cheek cell, you would take a piece of cotton wool and rub it on the inside of your cheek and the rub it on a microscope slide, the you add a little distilled water and then a drop of methylene blue. The final step you would take, is to cover the slide with a cover slip, then place it under the microscope.
Wiki User
∙ 16y ago
Wiki User
∙ 9y agoMethylene blue stain is a chemical compound. It is used for medical procedures like removing polyps. It can also stain the fibers of a nerve.
Wiki User
∙ 14y agoTo stain slides for cell identification.
Wiki User
∙ 11y agoMethylene blue is a chemical that stains a specimen dark blue. To do a Gram Stain, you need a different stain, like crystal violet.
Add your answer:
Earn +20 pts
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
Why cant you use methylene blue when staining onion cells?
Methelyne BLue is a great stain to use for onion cells. It is toxic, however, and in activities that involve young kids it is often substituted.
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Can methylene blue expire?
If it has a use by date you are supposed to observe that date. I personally would ignore the use by date and use it
What is the advantage of the gram stain over simple stains such as methylene blue?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Chemical used to make a specimen visible?
A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What kind of stain use in histology?
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Which substance can a student use to make the nuclei more visible?
methylene blue
Why cant you use methylene blue when staining onion cells?
Methelyne BLue is a great stain to use for onion cells. It is toxic, however, and in activities that involve young kids it is often substituted.
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Can methylene blue expire?
If it has a use by date you are supposed to observe that date. I personally would ignore the use by date and use it
