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Imagine a vessel where you are growing E. coli and after some time it reaches the highest density it can so it stops growing. Take half of that culture and put it into another vessel so now you have two vessels. Dilute them to the original volume with fresh nutrients etc. Those cultures will start to grown once more. Keep doing this over and over or however many times you need.

It is a way of getting more biomass from the original culture.

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12y ago

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