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The enzyme responsible for the color change of methylene blue is typically an oxidase, such as glucose oxidase or other redox enzymes. These enzymes facilitate the reduction of methylene blue, converting it from its oxidized blue form to a colorless leuco form. This color change occurs due to the transfer of electrons during the enzymatic reaction, effectively altering the dye's oxidation state and its light-absorbing properties. Such reactions are often employed in biochemical assays to indicate the presence of specific substrates or enzymatic activity.

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1mo ago

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