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Biuret reagent turns from light blue to purple in the presence of proteins or peptides, but it does not change color in the presence of fats. Fats and oils are not detected by biuret reagent.

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What color does starch turn mixed with biuret reagent and why?

Starch does not react with Biuret reagent, which is primarily used to test for proteins. When Biuret reagent is added to a solution containing proteins, it turns a purple color due to the formation of a complex between copper ions in the reagent and peptide bonds in proteins. Therefore, if starch is present, it will not cause any color change with Biuret; the solution will remain blue, indicating the absence of proteins.


What chemical reagent of protein?

Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.


What is the colour change in Protein when the Bluret test is performed?

When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.


What color does distilled water turn with Benedict's reagent?

The water turns a pale blue. In other words stating that it doesnt contain protein


To test wether a sample contains proteins what reagent should be used?

The Bradford reagent (Coomassie) is commonly used to detect if a sample contains protein. Coomassie will react with aromatic amino acids (phenylalanine, tyrosine and tryptophan) to turn from a dull red color to a bright blue color. This assay is dependant on the amount of aromatic amino acids present, but works well as a "quick and dirty" indicator of the presence of a protein. The bicinchonic acid assay (BCA assay), while more expensive than the Bradford assay, more accurately detects the presence of the peptide bond present in proteins, so it can be used to not only detect proteins which lack aromatic amino acid residues, but also can be used to more accurately determine the concentration of protein in a sample as not all proteins have the same amount of aromatic amino acids.

Related Questions

If biuret reagent turns purple what substance is present?

Proteins are present when biuret reagent turns purple. Biuret reagent reacts with peptide bonds in proteins, causing the color change.


What color does biuret reagent turn when peptides are present?

Biuret reagent turns purple when peptides are present. This color change is due to a complex formation between the peptides and copper ions in the reagent, which results in the purple color.


Does biuret's reagent turn lavender when heated?

No, biuret's reagent typically turns violet or pink when heated, not lavender. This color change is a characteristic reaction with proteins in which the copper ions in the reagent complex with peptide bonds, forming a colored complex.


When using Biuret reagent what indicates the presence of proteins?

Biuret solution turns a shade of purple if proteins are present. Specifically, it turns a shade of purple if peptide bonds are present. A peptide bond is the bond formed between amino acids.


What chemical reagent of protein?

Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.


What color does Biuret turn in the presence of protein?

purple


Why does biuret reagent turn the skin brownish purple?

Biuret reagent react with the skin proteins.


What is the essense of adding water to a biuret reagent do you have to add water to the reaction?

water turns blue


What is the colour change in Protein when the Bluret test is performed?

When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.


What is the result when albumin solution pepsin and HCl are mixed with biuret reagent?

When albumin solution, pepsin, and HCl are mixed with biuret reagent, the resulting solution would likely turn purple due to the presence of proteins in the albumin solution. The biuret reagent reacts with peptide bonds in proteins, forming a purple complex. Pepsin is a protease enzyme that can break down proteins, potentially affecting the intensity of the purple color.


When biuret reagent is added to an unknown colorless solution the solution turns light purple. this indicates the presence of which molecules?

process that release energy by breaking down food molecules in the presence of oxygen


What color does distilled water turn with Benedict's reagent?

The water turns a pale blue. In other words stating that it doesnt contain protein