Chromatography

The molecular formula of Schiff reagent is C20H15N3. It is a chemical reagent used for detecting the presence of aldehydes or ketones in organic compounds.

The solvent rises up the chromatography paper (blotting paper) by capillarity. When the solvent reaches the "spot" it dissolves the mixture of coloured chemicals. There is now a solution; this is a mixture of solutes dissolved in the solvent. The molecules of these different chemicals are all different sizes. The simple explanation is that the smallest solute molecules travel almost as quickly as the solvent molecules and so get carried to the top of the chromatogram. The largest solute molecules travel very slowly and stay near the bottom. So some of the coloured chemical travel further than others.

When the temperature is programmed to increase in Gas Chromatography, it is called temperature programming or temperature ramping. This technique involves gradually increasing the temperature during the analysis to separate compounds based on their boiling points and improve resolution.

Open tubular columns provide greater resolution in gas chromatography because they have a larger surface area for interaction between the sample components and the stationary phase, leading to better separation. Additionally, the lower mass transfer resistance in open tubular columns allows for faster analysis times and improved peak shape, resulting in higher resolution.

Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds.

no

Yes it can.Due to the little holes in the filter paper. The holes send through the clean water into a beaker.

No it won't. Look at the mixture of oil, water and food coloring.

Chromatography paper is also called filter paper because it is commonly used to separate mixtures of substances based on their different polarities as they move through the paper.

· Taking and giving sample incorrectly

· Missing quantity control by statistics

· Incorrect mixing which will give you a false data density, false integration start and stop because of poor peak detection, false base line positioning.

Ion exchange chromatography allows for high resolution separation of charged particles based on their charge properties. It is a versatile technique that can be used for separating a wide range of molecules, from small ions to proteins and nucleic acids. Additionally, it is relatively simple to perform and can be easily scaled up for large-scale purification processes.

Chromatography is used in CSI to separate and analyze the components of complex mixtures like blood, drugs, and fibers found at crime scenes. By identifying and comparing the unique chemical fingerprints of substances, chromatography helps forensic scientists link evidence to suspects, determine timelines, and solve crimes. Different types of chromatography, such as gas chromatography and liquid chromatography, can be utilized depending on the nature of the sample being analyzed.

Methyl orange is commonly used as an indicator in paper chromatography. By placing a drop of the methyl orange solution onto the paper and allowing it to dry, when the paper is placed in a solvent, the components will move up the paper at different rates based on their chemical properties. This allows for separation and identification of the components in the mixture.

When you have a gas or fluid composed of multiple compounds and/or elements. The components all weight different amounts and have different diffusion rates, so you can measure the bands thickness and location to get the parts of the compound. In addition, the colors of the materials are also used to figure the elements.

i think that you have to crush the skittles first (so that you can hardly see them) then filter the substance in then do the chromatography process. sorry if this isn't much help an it might be wrong so you will have to check i am at secondary school and throughout all of my years of being at school i never got taught if you can use chromatography on solids... oh well... why don't you try differant ways yourself i may answer your question

Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods.

Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.

Disadvantages of using an internal standard in gas chromatography include the need for additional sample processing steps, the potential for introducing errors during the mixing of the internal standard with the sample, and the possibility of the internal standard not behaving identically to the target analyte during the analysis.

the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes !

Hope this helped ;) xxx

Filter paper is used in chromatography to separate the components of a mixture based on their different rates of solubility and adsorption. The paper acts as the stationary phase, allowing the solvent to move the mixture components through it at different speeds. This separation allows for the visualization and characterization of individual components within the mixture.

The Rf value of decane will depend on the specific conditions of the chromatography experiment (type of solvent, type of stationary phase, etc). In general, decane is non-polar and tends to have a higher Rf value in non-polar solvents compared to polar solvents. Typically, the Rf value of decane in non-polar solvents is close to 1.

Analyzing the spectrum of a star, or galaxy, can provide some useful information, including the temperature of a star, whether a star or galaxy is moving towards us or away from us, and the chemical composition.

Chromatography could be used to separate the components of the reaction mixture and identify if aspirin is present by comparing the retention time of the product to that of a known aspirin standard. If the retention time matches, it indicates the presence of aspirin in the reaction mixture. Additionally, chromatography can help determine the purity of the aspirin product by analyzing the intensity of the peak corresponding to aspirin.

It refers to a set of technique used to separate different compounds. So involves separating chemicals and identifying them by color. Various chromatography products are used during the process.

Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a common type of chromatography used in organic chemistry for separating and analyzing compounds that can be vaporized without decomposition.

Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc

HIC and RPC are closely related techniques since both are based upon interactions between hydrophobic patches on the surface of biomolecules and the hydrophobic surfaces of a chromatography medium. However, in practice, the techniques are very different. The surface of an RPC medium is usually more hydrophobic than that of a HIC medium. This leads to stronger interactions that, for successful elution, must be reversed using non-polar, organic solvents such as acetonitrile or methanol. HIC media offer an alternative way of exploiting the hydrophobic properties of biomolecules by working in a more polar and less denaturing environment.

The selectivity factor in chromatography is a measure of how well a chromatographic method can separate two components of a mixture. It is calculated as the ratio of the retention factors of the two components. A higher selectivity factor indicates better separation between the two components.