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Molecular Biology
Molecular biology is the study of how molecules (biomolecules such as protein, DNA) communicate in the cell and how the field helps to study them in a external condition.
267 Questions
How many molecules does 14Fe have?
I was given this question by a teacher in chemistry and they told me that there was none. Hope that helped!
A DNA tiling array uses overlapping DNA fragments. Hundreds of thousands of genomic fragments can be can be spotted onto a glass slide. In most tiling arrays, RNA samples are still hybridized to the slide just like a cDNA array.
What are four ways transcription is repressed?
Transcriptional repression can happen by four different mechanisms.
The first method involves competition between transcriptional activators and repressors for binding to DNA. A DNA binding site can either be bound by a transcriptional activator or repressor. For example, binding of a transcriptional repressor to an enhancer element will block the binding of a transcriptional activator.
The second method gives rise to transcriptional activators and repressors to bind to different DNA sequences. Transcription can be blocked if the transcriptional repressor can physically bind to the activator and thereby blocks its activity.
The third method describes the direct physical contact between the transcriptional repressor and the mediator complex. The mediator complex is unable to phosphorylate RNA Polymerase II.
The fourth method is shown by the transcriptional repressor recruiting the HDAC proteins. As a result, the acetyl groups are removed from histone proteins. This increases the positive charge on the core histones. This tightens the grip they have on DNA.
How do the structures of the purines and pyrimidines increase the accuracy of DNA replication?
Accuracy or fidelity of replication doesnt depend on DNA bases, the fidelity or keeping the DNA as correct is accompanied by DNA polymerase enzyme!
How is digestion and separation are used in molecular biology?
Molecular biology is The science of the study of life phenomena at the molecular level. By studying the biological macromolecules (nucleic acid, protein) of the structure, function and biological synthesis to illustrate various aspects such as the essence of life.
If you want to know more about other knowledge of molecular biology, you can look to www.myelibrary.net, register it , it is for the majority of scientific research personnel to provide the site a lot of Chinese and foreign literature database data, will be helpful to you
What is the working principal behind RT PCR?
Reverse transcription polymerase chain reaction (RT-PCR), is a variant of polymerase chain reaction (PCR) commonly used in molecular biology to detect RNA expression. RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.
Even though both techniques, RT-PCR and PCR, produce multiple copies of a particular DNA through amplification, the applications of the two techniques are fundamentally different. The most common PCR technique is used to exponentially amplify target DNA sequences. Meanwhile, RT-PCR is used to clone expressed genes by reverse transcribing the RNA of interest into its DNA complement through the use of reverse transcriptase enzymes. Subsequently, the newly synthesized cDNA by RT-PCR is amplified using traditional PCR technique.
Usually, RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and researchers alike, but they are quite separate and distinct techniques.
When was Roche Institute of Molecular Biology created?
Roche Institute of Molecular Biology was created in 1967.
What antibody use in western blot technique?
Generally there are two antibodies used. Primary antibody which can bind specifically to the protein of interest. And a secondary antibody coupled with a detection system such as HRP that would bind the primary antibody and signals the presence of protein of interest.
Where does the RNA polymerase moves down?
RNA polymerase catalyze the synthesis of RNA by copying the DNA. It occurs in the 5' to 3' direction(moves down).
Yes. Everything that is bigger than molecules is made of molecules.
What is an RNA particle that causes disease?
A plant pathologist discovered an infectious RNA particle smaller than a virus that causes diseases in plants. He called it a viroid. Millions each year are lost by these viroids. These contain only RNA.
What are three-carbon molecules of pga are converted to energy-rich g3p sugar molecules by?
butt muffins
Which forces are involved in holding the drugs to the active site of enzymes?
Hydrogen bonding, ionic bonding, dipole-dipole interactions or van der Waals interaction.
What important clue from francklin's work helped Watson and crick develop their model of DNA?
Franklin's X-ray diffraction images provided crucial information about the structure of DNA, specifically indicating that it had a helical structure with a repeating pattern. This was key in guiding Watson and Crick to propose their double helix model of DNA.
