PCR was developed in 1984 by Kary Mullis.
DNA Polymerase.
DNA Polymerase is the enzyme which adds new nucleotides during replication.
This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.
DNA polymerase is a catalyst, by catalyzing the synthesis of new DNA by adding nucleotides to a preexisting chain. There are several different DNA polymerases, but DNA polymerase I and DNA polymerase II play the major roles in DNA replication.
RNA mRNA tRNA
DNA ligase. Apex
DNA Polymerase.
Alice Chien, et al. were the first to describe the Taqpolymerase in 1976.Then, Saiki et al. were the first to employ the polymerase in a published study.Chien A, Edgar DB, Trela JM. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 1976;127:1550-1557.Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-491.
DNA Polymerase is the enzyme which adds new nucleotides during replication.
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
DNA polymerase matches the bases on the parent strand.
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
DNA Polymerase III
dna polymerase
DNA polymerase is a catalyst, by catalyzing the synthesis of new DNA by adding nucleotides to a preexisting chain. There are several different DNA polymerases, but DNA polymerase I and DNA polymerase II play the major roles in DNA replication.
This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.
DNA polymerase :)