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Can you see spores in a gram stain preparation?
yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
How does culture age affect the results of a sproe stain?
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Are mosses spores or seeds?
Mosses reproduce by spores.
Why are spores so difficult to stain?
These bacteria that have flagella have to be handled more carefully than bacteria without flagella because the flagella could brake off the bacteria. Remember that some bacterial flagella are very small and delicate.
How does culture age affect results of a spore stain?
Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.
Can you see spores in a gram stain preparation?
yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
Of what use is the endospore stain?
The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
What kind of diseases can cabbage have?
Clubroot, Downy Mildew and Fusarium Wilt.
Why are spores more difficult to stain than vegetative cells?
Spores are very hard and dense, dye is not readily absorbed into the endospore. However, one method of staining is the Schaeffer and Fulton method. The stain is malachite green and the proper method entails preparing a heat fixed smear which is covered by a piece of blotting paper, and flooded with the dye. Wait 15 mins then remove blotting paper and wash. Counterstain with 0.5% Safrinin. Spores appear green.
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
How does culture age affect the results of a sproe stain?
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Why is it ot necessary to include a negative control for the spore staining procedures?
When you stain endospores, the control sample is the cell itself, since you are only looking at the spores, which are present inside the cell.
