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Why are spores so difficult to stain?

Updated: 8/11/2023
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Ambamstudee

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14y ago

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These bacteria that have flagella have to be handled more carefully than bacteria without flagella because the flagella could brake off the bacteria. Remember that some bacterial flagella are very small and delicate.

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13y ago
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Wiki User

14y ago

Spores have a more complex structure then cells (dense and multilayered) which makes them less permeable to stains.

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Q: Why are spores so difficult to stain?
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Related questions

Can you see spores in a gram stain preparation?

yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.


Why are spores more difficult to stain than vegetative cells?

Spores are very hard and dense, dye is not readily absorbed into the endospore. However, one method of staining is the Schaeffer and Fulton method. The stain is malachite green and the proper method entails preparing a heat fixed smear which is covered by a piece of blotting paper, and flooded with the dye. Wait 15 mins then remove blotting paper and wash. Counterstain with 0.5% Safrinin. Spores appear green.


Is it easy or difficult to destroy fungal spores?

it is difficult because as quick as your getti rid of the spores the bacteria will ammediatly grow back


What dye is used to stain endospores?

To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.


How does culture age affect results of a spore stain?

Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.


Why does mustard stain?

Mustard stains because it is a dye stain ,which makes it difficult to get out.


Can l stain on stain?

Yes, you can, but it's difficult to get a satisfactory appearance as it is now not soaking into the wood.


Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?

spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore


Of what use is the endospore stain?

The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.


Which stain is most difficult to remove?

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How does culture age affect the results of a sproe stain?

Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.


Does blood stain clothes?

Yes, blood stains clothes. It is a protein stain, and is difficult to remove. If you have a blood stain to remove, use cold water and pretreat it with OxyClean.