These bacteria that have flagella have to be handled more carefully than bacteria without flagella because the flagella could brake off the bacteria. Remember that some bacterial flagella are very small and delicate.
Spores have a more complex structure then cells (dense and multilayered) which makes them less permeable to stains.
yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
it is difficult because as quick as your getti rid of the spores the bacteria will ammediatly grow back
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Microspores develop inside the microsporangium
yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
Spores are very hard and dense, dye is not readily absorbed into the endospore. However, one method of staining is the Schaeffer and Fulton method. The stain is malachite green and the proper method entails preparing a heat fixed smear which is covered by a piece of blotting paper, and flooded with the dye. Wait 15 mins then remove blotting paper and wash. Counterstain with 0.5% Safrinin. Spores appear green.
it is difficult because as quick as your getti rid of the spores the bacteria will ammediatly grow back
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.
Mustard stains because it is a dye stain ,which makes it difficult to get out.
Yes, you can, but it's difficult to get a satisfactory appearance as it is now not soaking into the wood.
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
Red Wine
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Yes, blood stains clothes. It is a protein stain, and is difficult to remove. If you have a blood stain to remove, use cold water and pretreat it with OxyClean.