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Suppose a restriction enzyme recognizes the six-base sequence aagctt ttcgaa in a double strand of DNA between which two nucleotides on each strand would the enzyme have to cut to produce a fragment w?
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Why might a laboratory be using dideoxy nucleotides?
To sequence a DNA fragment
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
What cuts bacterial plasmids?
Enzymes called restriction endonucleases can cut plasmids. However, in order for a cut to be produced, the plasmid should contain a specific sequence of nucleotides called the restriction site
The kind of protiens produced in a cell are related to the?
sequence of nucleotides in the DNA
Why might a laboratory be using dideoxy nucleotides?
To sequence a DNA fragment
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
A molecule that cuts DNA molecules at a specific sequence of nucleotides?
A restriction enzyme
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
What cuts bacterial plasmids?
Enzymes called restriction endonucleases can cut plasmids. However, in order for a cut to be produced, the plasmid should contain a specific sequence of nucleotides called the restriction site
What are restricted enzymes used for?
Restriction enzymes are used in lab for cutting DNA sequences at specific sites.The RE used will cut only at its recognized site. expose the base sequence of a DNA fragment. they are enzymes isolated from bacteria.They are of two types.Restriction endonuclease & restriction exo nuclease.exonucleases remove nucleotides from the ends of the DNA .endonucleases make cuts at specific positions in the DNA
The sequence of what carries genetic information as an organism?
nucleotides
The kind of protiens produced in a cell are related to the?
sequence of nucleotides in the DNA
How she was able to add the HInd III restriction enzyme site in this position based on the result of her gel?
She was able to do so because the band pattern portrayed on the agarose gel shows exactly where the sequence AAGCTT was located on the DNA. Because of this, she was able to place the Hind III restriction enzyme right at that sequence since it was given that the enzyme recognizes that specific sequence.
How is a gene related to the sequence of nucleotides?
Genes are segments of DNA. DNA is made up of polymer of nucleotides joined together. When there is an alteration in the sequence of nucleotides, gene mutation occurs.
