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Why taq polymerase is strored at very low temperature as it is thermostable?
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
What is the name of the enzyme that match the DNA bases?
DNA polymerase matches the bases on the parent strand.
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
Is vent polymerase a thermostable enzyme?
Yes. Vent polymerase is thermostable and is commonly used for PCR
What is the significance of thermostable polymerase in DNA technology?
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
Why taq polymerase is strored at very low temperature as it is thermostable?
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Principle enzyme involved in DNA replication?
DNA Polymerase.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
What is the name of the enzyme that match the DNA bases?
DNA polymerase matches the bases on the parent strand.
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
