0
Why must the level of the solvent not reach the pencil line where the spots are?
Wiki User
∙ 11y ago
because the colours would mix together, making the results unreliable
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
Why must the level ofthe solvent not reach the pencil line where the spots are?
if it wouldn't be above the level of solvent the spots will run off
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
Why must the level ofthe solvent not reach the pencil line where the spots are?
if it wouldn't be above the level of solvent the spots will run off
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
When preparing to develop a TLC plate what is the appropriate spatial relationship between the level of solvent in the developing chamber and the spots on your TLC plate?
The spots on the TLC plate should be placed about a cm from the bottom of the plate. Then solvent should then fall about half-way between these spots and the bottom of the beaker.
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
Solvent capable of removing herb spots?
i have no idea
Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
Why should the amino acids spots not be submerged within the solvent?
I assume you are talking about thin-layer chromatography. If the spots are submerged in the solvent, they will dissolve into it and become so diluted that they will most likely be undetectable. Plus, they will elute as a band, not as a spot. Your solvent will also be contaminated.
What would be the consequence of having so much solvent in the developing chamber that it would cover the application?
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
What is solvent front in chromatogram HPLC?
It is to ensure that the spots of colouring are completely (or at least near that) separated into the different dyes that make them up. The further up the paper the solvent front is, the greater the separation of the spots. The different distances moved by the different dyes in proportion to the distance the solvent moved is known as the Rf value. For more information, type: What is chromatography? It might help.
