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Would a water solvent separate colors in an ink pen in paper chromatography?
Wiki User
∙ 14y ago
It is entirely dependent upon solubility. First, the individual dyes that make up the ink's final color must be soluble. Then, the dyes that are more soluble are able to stay dissolved in the water longer than those that are less soluble, therefore getting further up the paper.
Wiki User
∙ 14y ago
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What would the result be if while carrying out TLC chromatography you used a solvent of 200 is to 1 ethyl acetate and aqueous ammonia instead of 200 is to 1 ethyl acetate and acetic acid?
chromatography is when two substances separate. iprdufwjiwreusdoi
Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
Why is the mixture placed above the level of the solvent in chromatography?
If not, the mixture would just soak out into the liquid in the tank.
Why can the pigment spot on chromatography paper not be submerged in solvent?
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
What type of chromatography would be used to separate two proteins?
Ion-exchange chromatography would be used to separate two proteins.
What would the result be if while carrying out TLC chromatography you used a solvent of 200 is to 1 ethyl acetate and aqueous ammonia instead of 200 is to 1 ethyl acetate and acetic acid?
chromatography is when two substances separate. iprdufwjiwreusdoi
Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
Why is the mixture placed above the level of the solvent in chromatography?
If not, the mixture would just soak out into the liquid in the tank.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
Why can the pigment spot on chromatography paper not be submerged in solvent?
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Can TLC or paper chromatography be used to separate and identify very volatile substances?
Thin layer chromatography is not a method that can be used for very volatile substances. When a thin layer plate is removed from a developing tank, one needs to evaporate off the developing solvent, which is sometimes done by heating the plate or moving it thorugh a stream of air. Any volatile components on the plate would be removed at the same time. In order to separate and identify volatile compounds, you'd use hplc or gc (high performance liquid or gas chromatography.
Would RF values of amino acid you use in paper chromatography be the same if you use another solvent system?
Rf value is the ratio of distanced covered by solute by distanced covered by solvent.
Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
Eluotropic series mean in relation to chromatography?
The eluotropic series in chromatography refers to a list of solvents ranked based on their ability to elute (separate) components of a mixture from the stationary phase. Solvents higher in the eluotropic series are more polar and have stronger interactions with the stationary phase, thus making it easier for components to move through the column. The eluotropic series is useful in selecting appropriate solvents for different chromatographic separations.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
