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Where do the shorter DNA bands end up reaching at the completion of gel electrophoresis?
Wiki User
∙ 12y ago
the smallest DNA fragments are observed by a process called elcrophoresis where the DNA fragmnets placed on this gel will migrate according to their lenght so the smallest fragment will migrate the fastest and they will be found at the bottom .
Wiki User
∙ 14y ago
Wiki User
∙ 12y agoElectrophoresis should be terminated before DNA has migrated off of the end of the tray. Fluorescent dyes like ethidium bromide (use great caution, as this is a mutagen) can be used to monitor how far DNA moves down the gel.
Shorter segments of DNA move the farthest, as they are most easily able to move through the tight pores present in the agarose gel. The DNA bands that have moved closest to the positive electrode are the smallest. Reference DNA can be placed in a separate lane of the gel tray to somewhat accurately determine the size of fragments present.
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What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
Is DNA smaller or bigger than a protein?
Let's put it this way, we know that electrophoresis is a test for the sizes of the fragments of DNA molecules while SDS-page is a test of the size of protein molecules. If you use electrophoresis to test the differences of protein, there will not be any bands as all the protein will travel to the end of SDS-page. Therefore, we can conclude that the pores of electrophoresis is much more larger than SDS-page. Since electrophoresis has larger pores than SDS-page, it also shows that overall DNA is larger than protein in size.
The sequence of a DNA fragment can be determined by observing the order of the fluorescent bands on the electrophoresis gel?
There is no answer you are all being lied to you are all in the matrix this is the matrix this is me morphius and this is the matrix do you take the blue pill or te red pill
How can a mutation that alters a recognition site be detected in gel electrophoresis?
The point mutation has to result in either the removal of a restriction site of the restriction enzymes or the formation of a new one, such that the bands of mutated DNA that form after performing gel electrophoresis are different from the normal one. So a difference in banding patterns would mean that there is a point mutation.
Which technique was use to produce these bands?
Gel electrophoresis
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Are restriction enzymes used in gel electrophoresis?
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
How do you avoid smearing of DNA bands in gel electrophoresis?
Make sure the voltage does not run over 200.
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
Why does the the florescense appear in the chaneels only n not in the form of moving bands While performing Gel electrophoresis For DNA detection?
Only the bands should show fluorescence, you must be doing the technique wrong..
DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?
DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.
What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?
They would be the same since Dolly is clone.
What method could you use to further separate two bands from the same protein fraction after SDS-PAGE?
Electrophoresis is the method that could be used to further separate two bands from the same protein fraction after SDS-PAGE.
