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Biochemistry

Biochemistry is the study of the structure, composition and chemical processes in all living organisms. It covers the structures and functions of various cellular components, including proteins, carbohydrates, nucleic acids, lipids, and other biomolecules.

8,065 Questions

What environment factors can affect the activity of an enzyme?

Factors such as temperature, pH levels, substrate concentration, and the presence of inhibitors or activators can affect the activity of an enzyme. Changes in these environmental conditions can alter the enzyme's structure, ultimately impacting its ability to catalyze reactions efficiently.

What happens during translation and where does it occur?

Translation is the process where messenger RNA (mRNA) is decoded by ribosomes to synthesize proteins. It occurs in the cytoplasm of a cell, on ribosomes, where transfer RNA (tRNA) molecules bring amino acids to the ribosome based on the codons on the mRNA, leading to the assembly of the protein chain.

How ketogenic amino acid converted to glucose?

Ketogenic amino acids are converted into acetyl-CoA, which can enter the Krebs cycle to produce ATP or undergo ketogenesis to produce ketone bodies. Acetyl-CoA cannot be converted back into glucose directly, as it bypasses the steps of gluconeogenesis. Glucose can be synthesized from non-ketogenic amino acids or other substrates through gluconeogenesis in the liver.

What did hershey and chase find through their experiment with virus-infected bacteria?

The idea of their experiment was to determine what kind of macromolecule was the genetic material, DNA or proteins. Bacteriophages, viruses that infect bacteria, were known to carry all of the information needed to make themselves, but using the genetic machinery of their hosts. Based on this, they were used as a simple system that should be governed by the same principals as the genetic information of the bacteria itself.

At the time it was known that nearly all phosphorus in macromolecules is found in nucleic acid, and nearly all sulfur is found in proteins (methionine and cycteine). So if you grow cells in radioactive isotopes of phosphorus, the nucleic acids (e.g. DNA) become radioactive, and if you grow cells in radioactive sulfur, proteins become radioactive.

Sorry, there's more. Folks studying infection of bacteria by phage found that most of the virus was a "package" that never entered the cell. So if they mixed phage with bacteria and waited 15 minutes and then put the mix in a blender for a few seconds, the packages that had been attached to the outside of the bacteria get ripped off and can be searated from the bacteria. While the packages are no longer attached to the bacteria, the bacteria are already infected, so the virus genes must already be inside.

Now the experiment: Hershey and Chase infected bacteria with phage with racdioactive proteins (sulfur) or in a separate experiment radioactive DNA (phosphorus). The bacteria was mixed with virus for 15 minutes, then whoosh, frappe! They then separated the bacteria from the viruses that had not infected bacteria and empty packages, and asked what kind of radioactive macromoleules were able to get inside the bacteria following the short incubation.

In the sulfur experiment , the bacteria were NOT radioactive so proteins appeared to not enter the bacteria during this short time frame. In the phosphorus experiment, the bacteria became radioactive after a short incubation, meaning the DNA of the phage had entered the bacteria in the same time frame as the genetic material. This was consistent with DNA being the genetic material of bacteriophage.

Why does the movement of chloride ions causes water to pass out of the cells into the mucus?

The chloride ions have made the mucus more concentrated of the ions. The water moves from a high concentration of water in the cells to a low concentration of water which is in the mucus; in order to dilute the more concentrated solution. It travels by osmosis through a partially permeable membrane.

What is larger a monosaccharde a disaccharide or a polysaccharide?

1-3 largest to smallest

  1. Polysaccharde (It's a long chain of monosaccharides)
  2. Disaccharide ("Di" is Greek for 2. It's made of 2 monosaccharides)
  3. Monosaccharide (simple sugar like glucose, galactose, or fructose)

How does a blood serum enzyme test diagnose myopathy?

A blood serum enzyme test can diagnose myopathy by measuring the levels of enzymes that leak into the bloodstream when muscle cells are damaged. Elevated levels of enzymes such as creatine kinase (CK) can indicate muscle damage, which is common in myopathy. By analyzing these enzyme levels, healthcare providers can assess the severity of muscle damage and monitor response to treatment.

How many moles of H2O are produced by the complete combustion of methane?

For the complete combustion of methane (CH4), the balanced chemical equation is:

CH4 + 2O2 -> CO2 + 2H2O

This equation shows that for every mole of methane that reacts, 2 moles of water (H2O) are produced.

Do electrons slide along the electron transport chain and onto ATP molecules?

They do slide along the electron transport chain, but not onto molecules of ATP. The motive power of these electrons are used to pump protons into the outer lumen of the mitochondria. There the protons build up until there is a powerful enough gradient so that they come down that gradient and through the ATP synthase, a rotary enzyme that uses the flow of protons to attach a phosphate group onto ADP and thus ATP is synthesized.

The electrons emerge from the last electron transport complex and there oxygen takes them up with the spent protons and water results.

Is copper important to living things?

Yes, because copper helps develop human bones, connective tissue, and nerve coverings. It also helps make electricity, airplanes, cars, tools, plumbing pipes, weapons, and is used to make pennies.

What is qrxn the symbol for?

"qrxn" typically represents the heat transferred during a chemical reaction.

Why does nad become nadh plus h instead of just nadh during glycolysis?

During glycolysis, NAD+ acts as an electron carrier molecule. It accepts two electrons and a proton to form NADH. This is important for the oxidation-reduction reactions that occur during glycolysis, allowing for the transfer of electrons and the generation of ATP.

How is the enzyme glucose isomerase used in the soft -drink industry?

Glucose isomerase is used in the soft-drink industry to convert glucose into fructose, which is sweeter and more soluble. This process, known as isomerization, helps create high-fructose corn syrup (HFCS), a common sweetener in soft drinks. By using glucose isomerase, manufacturers can optimize sweetness, texture, and flavor in their products.

WHat are the Krebs cycle reactants?

This cycle occurs only in the presence of oxygen. It takes place in the mitochondria.

The oxidation of pyruvic acid into CO2 and water is called Krebs cycle:

Pyruvic acid +CoA --------> Acetyl CoA +CO2. This reaction is catalyzed by a set of enzymes called pyruvic acid dehydrogenase. In this reaction, 2 hydrogen atoms and one CO2 molecule are removed.

The 2 hydrogen atoms are accepted by NAD and NAD is converted into NADH.

Pyruvic acid and CoA are the reactants. The products are 2 H+ and CO2 and Acetyl CoA.

What is the basic unit of dna that is made up of a phosphate a sugar and a nitrogenous base?

The basic unit of DNA is a nucleotide, consisting of a phosphate group, a sugar molecule (deoxyribose in DNA), and a nitrogenous base (adenine, thymine, guanine, or cytosine). These nucleotides link together to form the DNA backbone through phosphodiester bonds.

A reaction that requires oxygen is called?

While many reactions involve oxygen, only combustion reactions require oxygen as a reactant.

What are some good journal sources of information on antihypertensive drugs?

Belden, Heidi. "Debate Continues Over Best Drug for Hypertension." Drug Topics (April 21, 2003): 32. Mechcatie, Elizabeth. "Genetics Will Guide Prescribing for Hypertension: Genotype Predicts Response to Drug." Internal Medicine News (July 1, 2003)

What hormone does the parathyroid glad produce?

The parathyroid gland produces parathyroid hormone (PTH), which helps regulate calcium levels in the blood by stimulating the release of calcium from bones, increasing calcium absorption in the intestines, and reducing calcium excretion by the kidneys.

What is a protein that catalyze chemical reaction for organism?

An enzyme is a protein that catalyzes chemical reactions in organisms by lowering the activation energy required for the reaction to occur. Enzymes are vital for various biological processes such as metabolism, growth, and repair.

What is the importance of enzymes to biological systems?

Enzymes play a critical role in biological systems by speeding up chemical reactions essential for life processes such as metabolism and growth. They enable processes to occur at a faster rate and under milder conditions than would otherwise be possible. Enzymes also help to regulate and control cellular functions, ensuring that biochemical reactions occur in a precise and coordinated manner.

How many fatty acids and glycerol do triglycerides contain?

The triglyceride molecule consists of three lipids bonded one each to the three Carbons of glycerol.

Why do you need dissolved gases in water?

Dissolved gases in water are important for aquatic organisms to respire and survive. Oxygen, for instance, is necessary for the survival of fish and other aquatic organisms, while carbon dioxide is essential for photosynthesis in aquatic plants. Additionally, gases like nitrogen help support the ecosystem by providing essential nutrients for growth.

What is a three base segement of tRNA?

A three-base segment of tRNA is called an anticodon. The anticodon is complementary to the mRNA codon during translation and helps to ensure the correct pairing of amino acids to the mRNA sequence. This is a fundamental mechanism in protein synthesis.

Why doesn't toasted wheat germ work for DNA extraction?

Toasted wheat germ contains compounds that can interfere with the DNA extraction process, such as fats and proteins. These compounds can inhibit DNA isolation and purification by binding to or degrading the DNA molecules. It is recommended to use fresh, unprocessed wheat germ for successful DNA extraction.