How does restriction endonucleases work?
Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. While recognition sequences vary between 4 and 8 nucleotides, many of them are palindromic, which correspond to nitrogenous base sequences that read the same backwards and forwards. In theory, there are two types of palindromic sequences that can be possible in DNA. The mirror-likepalindrome is similar to those found in ordinary text, in which a sequence reads the same forward and backwards on a single strand of DNA strand, as in GTAATG. The inverted repeat palindrome is also a sequence that reads the same forward and backwards, but the forward and backward sequences are found in complementary DNA strands (i.e., of double-stranded DNA), as in GTATAC (GTATAC being complementary to CATATG). Inverted repeat palindromes are more common and have greater biological importance than mirror-like palindromes.
What are the disadvantages for global food supply in biotechnology?
The prevalence of inefficient and non-nutritious food sources by large companies for profit. Large companies have the capital to pursue biotechnology but the result can be the domination of a market by a inefficient crop like america's corn production. It is profit motivated not globally responsible.
What are worries about biotechnology?
Some concerns about biotechnology include ethical implications of genetically modifying organisms, potential environmental impacts of modified crops, and worries about the long-term effects on biodiversity and ecosystem balance. Additionally, there are concerns about the concentration of power and control in the hands of a few big companies in the biotechnology industry.
Can a maths student go for biotechnology?
Yes, a math student can pursue a career in biotechnology. Many aspects of biotechnology involve mathematical concepts such as modeling biological systems, analyzing data from experiments, and optimizing processes in biotechnology. A strong background in mathematics can be beneficial in understanding and solving complex problems in biotechnology.
Which method can be used to gain the appropriate data for protein size and shape determinations?
This question was posed to me in my college biotechnology class this week. These are the answers that I have found. The only thing that a biotechnologist would use is the X-ray crystallography. Because it gives all the data needed to determine the size and shape of proteins.
I do hope that helped.
What type of functional RNA is a primary component of the structures required for protein synthesis?
Transfer RNA (tRNA) is a primary component of the structures required for protein synthesis. It is responsible for carrying specific amino acids to the ribosome during the process of translation in protein synthesis.
Is biotechnology is a good career option?
Biotechnology can be a good career option for individuals interested in science, research, and innovation. It offers opportunities to work in diverse fields such as healthcare, agriculture, and environmental science. With advancements in technology and growing demand for biotech solutions, it can provide a fulfilling and impactful career path.
What are two examples of traditional biotechnology?
Fermentation is a traditional biotechnology technique used to produce products like bread, beer, and yogurt. Another example is selective breeding, where desirable traits in plants or animals are deliberately bred together to create desired outcomes like disease resistance or increased crop yield.
Shuttle vectors are DNA plasmids that can replicate in multiple host organisms, such as bacteria and yeast. They have origins of replication and selection markers for both hosts, allowing researchers to move genetic material between different organisms for studies or applications in biotechnology. Shuttle vectors are valuable tools in molecular biology for studying gene function and expression across different systems.
Are cloned individuals infertile?
Cloned individuals are not necessarily infertile, as their ability to reproduce depends on various factors such as the cloning method used and any genetic abnormalities that may have occurred during the cloning process. In some cases, clones may have reduced fertility or health issues that could affect their ability to reproduce normally.
How does cloning effect humans?
Cloning in humans is a complex and controversial issue with ethical, legal, and social implications. While human cloning has not been successfully achieved for reproductive purposes, it may have potential benefits in research and therapeutic applications. However, there are concerns about safety, genetic variability, and potential misuse of cloning technology.
Why blue brain is called blue brain?
The Blue Brain Project is named after the color blue because blue is often associated with intelligence, creativity, and calmness in Western culture. The project aims to create biologically detailed digital reconstructions and simulations of the brain to deepen our understanding of brain function and create potential applications for artificial intelligence.
Why would you want to use biotechnology to make a product instead of making it in a laboratory?
Biotechnology allows for the use of living organisms, such as bacteria or yeast, to produce a product, which can be more cost-effective and sustainable compared to traditional laboratory synthesis methods. Additionally, biotechnology can often generate higher yields and purer forms of the desired product.
Why should there be more laws on biotechnology?
More laws on biotechnology can help regulate the ethical and safe use of biotechnological advancements, protect consumers from potential risks associated with genetically modified organisms, and ensure that research and development are conducted responsibly to prevent negative impacts on the environment and public health.
What hormone directly activated vir gene in agrobacterium Ti plasmid?
The hormone that directly activates the vir gene in Agrobacterium Ti plasmid is acetosyringone. It induces the expression of the vir genes, triggering the transfer of T-DNA from Agrobacterium to plant cells.
What is the difference between solid state and submerged fermentation?
Solid-state fermentation involves the growth of microorganisms on solid substrates (e.g., agricultural residues), usually in the absence of free-flowing water. In contrast, submerged fermentation occurs in liquid media where the microorganisms are submerged and suspended in the aqueous environment. Solid-state fermentation is typically used for the production of enzymes and organic acids, while submerged fermentation is common for the production of antibiotics and biofuels.
The size and charge of a protein (usually in kDa)
The process involves loading a sample with different proteins and separating them out based on size and charge. The bands that will appear will be the monomer of the protein (1 subunit) as the buffers typically used will typically be ionic in character and compete with the different subunits for ionic binding, resulting in the appearance of only that 1 subunit. For example Hemoglobin is a 64 kDa tetramer protein with 4 subunits (2 alpha, 2 beta) we would expect to see 2 bands, one for each of the 2 different subunits.
What the second step in the Polymerase chain reaction process?
The second step in the Polymerase chain reaction (PCR) process is annealing. During annealing, the temperature is lowered to allow the primers to bind to the DNA template strands. This facilitates the specific targeting of the region to be amplified.
Are bacteria the biggest micro organisms?
No, bacteria are not the biggest microorganisms. Some examples of larger microorganisms include fungi and protists. These microorganisms can range in size from a few micrometers to several millimeters.
How does the polymerase chain reaction make DNA more reliable?
The polymerase chain reaction (PCR) amplifies specific regions of DNA, making them easier to detect and study. By creating millions of copies of a target DNA sequence, PCR increases the reliability of detecting mutations or variations in the DNA. This increased specificity and abundance of DNA copies make PCR a valuable tool in various genetic analyses.
What do other organisms have that prokaryotes don't?
Prokaryotes differ with other organisms in
they don't have definite nucleus, nuclear envelop is absent. nucleolus is absent. no mitosis instead they show amitosis mediated by divisomes ( septasome ) which include the protein FtsZ ( filaments that are temperature sensitive Z ).
What are base pairs in biotechnology?
In biotechnology, base pairs refer to the complementary pairing of nitrogenous bases in DNA molecules. Adenine pairs with thymine, and guanine pairs with cytosine. Understanding base pairs is crucial for techniques like PCR and DNA sequencing.
Does a cloning vector contain promoter region?
Yes, a cloning vector can contain a promoter region. A promoter is a DNA sequence that initiates transcription of a particular gene, so cloning vectors can include a promoter to drive the expression of the inserted gene in the host organism.
What do you find interesting about biotechnology?
I find the way biotechnology can be used to improve human health, agriculture, and the environment fascinating. The potential for developing new treatments for diseases, creating genetically modified crops, and cleaning up pollution using biotechnological tools is exciting and holds great promise for the future.
Biotechnology can be expensive due to the cutting-edge technology, research and development costs, and regulatory requirements involved in creating new products or therapies. However, the cost can vary based on the specific application or project within the field of biotechnology.