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Chromatography

Chromatography is a set of techniques used to separate mixtures in a chemistry lab setting. The word chromatography means "color writing" and some of the first experiments in chromatography involved separating chlorophyll in plants.

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Why is gas-liquid chromatography also called vapor-phase chromatography?

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Asked by Wiki User

Gas-liquid chromatography is also called vapor-phase chromatography because it involves the separation of components of a sample based on their volatility in the vapor phase. In this technique, a gas (typically an inert carrier gas) is used to carry the sample through a liquid stationary phase, where separation occurs based on differences in partitioning between the gas and liquid phases. By using a volatile mobile phase, gas-liquid chromatography allows for the analysis of compounds with relatively low boiling points.

What is function of butanol in paper chromatography?

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Asked by Wiki User

Butanol is used as a solvent in paper chromatography because it can dissolve a wide range of compounds. It helps to carry the sample and allow it to migrate up the paper. Butanol also helps in separating the components of the sample by interacting differently with different compounds.

How high-performace liquid chromatography works?

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Asked by Wiki User

High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.

Eluotropic series mean in relation to chromatography?

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Asked by Wiki User

The eluotropic series in chromatography refers to a list of solvents ranked based on their ability to elute (separate) components of a mixture from the stationary phase. Solvents higher in the eluotropic series are more polar and have stronger interactions with the stationary phase, thus making it easier for components to move through the column. The eluotropic series is useful in selecting appropriate solvents for different chromatographic separations.

How is chromatography a analytical tool?

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Asked by Jonava

Chromatography is an analytical tool used to separate and analyze complex mixtures. It works based on the principle that different components in a mixture will move at different rates through a stationary phase when subjected to a mobile phase. By analyzing the resulting separation pattern, chromatography can provide valuable information about the composition and identity of the mixture being analyzed.

Would you expect the Rf value of a pigment to be the same if a different solvent were used Explain?

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Asked by Wiki User

The Rf value would not be the same for every solvent as there are factors that allow each solvent to be unique. The attractive force, particle size and solubility of each solvent will create different results each time.

How does a kidney remove wastes and keep fluids and salts in balance?

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Asked by Wiki User

can you explain how the kidneys remove wastes and keep fluids and salts in balance?

What is the value of a Chromograph by artist RF McIntyre?

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Asked by PontiacMi

Many of the pieces are valued at a price close to $10 to $15 each, The amount will vary depending upon the exact piece and the condition that it is in.

Why was Bohr's model called a planetary model?

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Asked by Wiki User

Because it postulates that the nuclei is in the center and electrons are spinning around the nuclei on its orbitals which is very similar to the planetary model of our solar system.

What are the similarities between paper chromatography and electrophoresis?

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Asked by Wiki User

Chromatography is a collective term for a family of lab techniques for the separation of the mixtures.It involves a passing a mixture dissolved in a mobile phase through a stationary phase.

Electrophoresis is the process by which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them. Each kind of molecule travels through the medium at a different rate, depending on its electrical charge and molecular size

In the electrophoresis techniques electricity is required and positive charge goes to the cathode whereas the negative charges goes to the anode (opposite charges attraction)

but in Chromatography there is no need for the current or electricity .

What is the general purpose of candy chromatography?

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Asked by Wiki User

The purpose is to separated the candy colors and identify them.

What are the disadvantages of an internal standard in gas chromatography?

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Asked by Wiki User

It has a poor efficiency for non-routine analysis. (you need to create an internal standard plot for each different ion you are measuring).

What is an Rf factor and how is it calculated?

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Asked by Wiki User

The Rf value is the "ratio to the front." Hence the R and the f. It is defined as the ration of the distance traveled by a spot (measured from the center) to the distance traveled by the solvent.

What is a chromatography absorber?

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Asked by Wiki User

Adsorptive chromatography is an analytical technique used for the chemical separation of mixtures and substances. The technique depends on the principle of selective adsorption (not to be confused with absorption), a type of adhesion.

What is the cost of chromatography?

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Asked by Wiki User

i honestly don't know I'm guessing $40-$100

How is chromatography useful for the police?

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Asked by Wiki User

search for it in a book just kidding its is when bobshaqalqa comes alive

Iupac definition of chromatography?

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Asked by Wiki User

"Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary while the other moves in a definite direction."

How is paper chromatography used in everyday life?

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Asked by Wiki User

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.

What are two industries that use chromatography?

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Asked by Wiki User

Chromatography is used in medical research and also scientific testing of soil & water for chemical contamination.

What is the difference between the stationary phase and the mobile phase in chromatography?

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Asked by Wiki User

stationary phase stays at the bottom of the paper chromatography while mobile phase is moving on the stationary phase and move on stationary phase till it gets its right place on the top of the paper or somwhere else.