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Chromatography

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Parent Category: Chemistry
Chromatography is a set of techniques used to separate mixtures in a chemistry lab setting. The word chromatography means "color writing" and some of the first experiments in chromatography involved separating chlorophyll in plants.
It is the separation of two or more substances in a solvent. Chromatography is a separation technique based on the different interactions of the compounds with the two phases, a mobile phase and a stationary phase, as the compounds travel through the supporting medium. The mobile phase is the ink s…
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Assuming it isn't waste heat from the surroundings, friction is the likely source, either with the sides of the containing vessels or from the pressure of compaction.
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Mass spec is use to further confirm your sample analyzed. Mass spectroscopy breaks down the sample into its components. It does this by bombarding the molecule with some type of particle (either alpha or beta... or i may be way off). This will break down the sample into certain weights. The mass spe…
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finding concentrations gas chromatogram of gasoline ion chromatogram of orange juice each peak corresponds to a separate component in the mixture area of each peak is proportional to concentration chemical fingerprinting species identification "killer" bees can be distinguished from nati…
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make sure that the solvent front does not go above the top of the paper.
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Many of the pieces are valued at a price close to $10 to $15 each, The amount will vary depending upon the exact piece and the condition that it is in.
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The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
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It's less expensive to perform, and you can do "two way" chromatography by running a chromatograph in one solvent, letting the paper dry, turning it 90 degrees and running a second chromatograph in a different solvent. Many times paper chromatography is used to visualize separate components of a s…
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Chromatography is used in forensic science. It seperates ink and can remeble links with criminals helping to solve cases. Answer: Chromatography is used in pharmaceutical industries for the synthesis of various drugs to check whether the product is synthesized from the reactants or not. It is used…
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It is used for filter the solution.
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Answer Gel filtration or gel permeation is a process by which molecules can be separated according to their size(molecular weight) and sometimes shape. small molecules get trapped and slowed down in the pores of the beads but large molecules simply flow down the column..therefore larger molecules…
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If the spot is too big, they may overlap with other spots.
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Answer It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
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Answer The size of the DNA determines the amount of distance traveled. Answer: Gel permeation Chromatography consists of a solvent reservoir, pump, polystyrene based column, injector, detector & data system.
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is that it can usually be scaled to the project at hand. This is especially useful if one is trying to separate .. By amanjot kaur ..
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Yes. Due to many factors. Evaporation, Particle sizes, and even color and shape.
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u have to see the ink that comes and see which 2 colours are same and next to each itherrThat is for paper chromatography!In gas chromatography you determine the concentration of the compound of interest in the sample by determining the area under the peak (AUC). The bigger the peak the higher the c…
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In liquid chromatography, the separation phase is a liquid and can either be done using column or plane chromatography. Column chromatography refers to the chromatographic bed shape and can be done with either a solid or liquid phase.
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because it will just end up dissolving in to the solvent and run of the paper before it even gets a chance to soak it's way up the paper.
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Chromatography is used to analyze , identify, purify & quantify the compounds.
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Answer:The factors which affect the chromatography are: Temperature Pressure Flow rate of mobile phase Sample preparation
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pigments are the molecules in the colours
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No, the solubility of the pigment molecule in its organic solvent is a factor involved in the separation of pigments. More soluble molecules move faster and generally travel further. Chlorophyll B is a great example since its only soluble in fat solutions.
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The first chromatography used was with polar stationary phase and non polar mobile phase, called normal phase. So, later when this was reversed by using polar mobile phase and non polar stationary phase was called reversed phase. Although reversed phase implies that it is less used, it is not the ca…
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It is used to separate different substances in a compound based on the difference in their solubility in water.
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Thin Layer Chromatography (TLC) is a method of separating the components that are in a sample of a mixture.
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Chromatography is used in blood to determine whether it is contaminated with alcohol and things like that. It can be also used to find out if cocaine etc. is in your urine and lead in water. This uses a colour chart to see what chemicals have been used and added up make a compound, then you can fin…
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acetone or butanol That totally depends on the solute you are trying to separate. Methylene Chloride generally works much better as an all around solvent due to its mild polarity and organic functional groups. Acetone won't work at all if you have 2 polar solutes. They will travel with the solvent …
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Chromatography can be used in all sorts of places here are a few:*For detectives*Food colouring *colour feltipsthis can separate a colour in to 2 colours whih it is made up of.=)
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Paper chromatography is a simple chromatography technique which has many applications. Its main advantage is that it is not very expensive to perform, and provides clear results. Given below are some important uses of paper chromatography. Separating Colored Pigments Paper chromatography is an effec…
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spots are often faint, and TLC is difficult to reproduce. NOT typically automated frm nisha ------------------------------------------------------------------------------------------------- just to improve your peice of work i have added more points on liquid chromatography Over-large Spots Une…
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Assay is the quantitative determination of the amount of a component in a sample. Purity is the determination of the impurities in the sample. It can be quantitative, semi-quantitative or qualitative.For example : if you dissolve 4 grams of compound A (with no impurities in it) in 6 grams of water, …
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When a solvent diffuses, it 'drags' whatever is dissolved in it along with it. The further it d\e same, the different dissolved materials sort out as different stripes or peaks and the banding can t\tography
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you can use to see what colors are in pen ink Answer: There are several types of chromatography: Gas ChromatographyLiquid ChromatographyIon Exchange ChromatographyAffinity Chromatography All allow the analysis of a solution by splitting its component parts into discernible fractions. This is useful …
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well first u do the whole chromatography thing. then u divide the solvent(distance the water traveled) by the distance the green tea components traveled alone. Then u should get you answer and should be between 0-1. Have a nice day;)
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Answer there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography: liquid chromatography gas chromatography
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The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
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TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a li…
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There are no units because it is simply a ratio
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Potency is a measure of the activity of a drug in a biological system & assay is analysis of drug to determine the presence, absence, or quantity of one or more components. A purity test is to ensure that all the analytical procedures performed allow an accurate statement of the content of impu…
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No it is compound.
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These are some disadvantages of using paper chromatography: 1. It can be used as a preparative technique because we can't aplly a large sample quantity; 2. It can't be used in quantitative analysis; 3. and doesn't allow the separation of complex mixtures. 4.this is one of oldest method
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Gas liquid chromatography specifically sepatates lipids.
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In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.
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They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
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atoms make bonds because each atom wnats to have a full valence shelll of electrons (they want eight electrons in their outermost shell). all of the elements, except for the noble gases, do not have a full octet (8 electrons in the outermost shell). thegroup one elements such as Na have one electron…
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Rf values are used mainly for simplicity. These values are generally easier to use to obtain percentages between a couple objects instead of using values that are already established.
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It is the rate of flow for each color. It is calculated by taking the value for the measured from the initial color mark to the solvent front (value A) and then the distance from the initial spot to each of the centers of the color marks separated (value B). You then divide B by A. For example, sol…
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The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.
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it helps because you can test experiments and also help solve the crime
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a moving or mobile phase is a mixture you want to separate , dissolved in a solvent.
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i think its blogspot is a good informational page for chromatography method EDIT: http://www.chromatography-online.org/ is a resource that has been doing well with me
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The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which invol…
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Paper chromatography is an analytical method of separating mixtures into their constituents. In the ascending technique, the separation of the mixture is against gravity.
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because it has capillary action
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1.Rate of flow of the solvent. 2.Strength of absorption & distribution of substance of the mixture on the stationary phase.
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GCMS involves running the sample through a mass spectrometer following the data received from chromatography. MS fragments the analytes to show patterns specific to the analyte (and the ionization technique and sector powers) therefore allowing the analyte to be identified. GC is used to separate al…
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Use mobilephase as diluent
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The principle behind Chromatography is : The rate of migration of the solute depends upon the rate of interaction of the solute with the two phases, one being the mobile phase and the other stationary phase as the compounds travel through the supporting medium.
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Chromatography is a collective term for a family of lab techniques for the separation of the mixtures.It involves a passing a mixture dissolved in a mobile phase through a stationary phase. Electrophoresis is the process by which molecules (such as proteins, DNA, or RNA fragments) can be separated …
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The biggest disadvantage in using paper chromatography is the time it takes to properly prepare and use. The second disadvantage is the small amounts of compounds that need to be handled.
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The principle behind Chromatography is : The rate of migration of the solute depends upon the rate of interaction of the solute with the two phases, one being the mobile phase and the other stationary phase as the compounds travel through the supporting medium.
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Inflation is considered to be undesirable because it arbitrarily redistributes wealth and real income. It also causes consumers to pay more for goods and services and causes the value of the dollar to go down.
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Chromatography is a tool where it makes it easier to analyze the DNA of a suspect and to match that analysis with any previously recorded DNA samples on file or what DNA was found at the crime scene. Chromatography is basically a separation technique based on the interactions of two different compou…
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Like all molecules, a molecule of H20 is in constant motion; 'hot' molecules move faster than 'cold' molecules. If the molecules move slowly enough the substance appears stationary to us (frozen water) and if they move quickly enough they will expand to fill their container (vapor or gas water.)
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Gas chromatography (GC) does not necessarily work in a specific way.Gas chromatography (GC), is used in analytical chemistry toseparate and analyze compounds that can be vaporized without decomposition test the purity of a particular substanceseparate the different components of a mixturedetermine t…
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Some drawbacks of gas chromatography include: The samples analysed are limited to those that are volatile or can be made volatile (reaction to form a volatile derivative). The samples must be thermally stable to prevent degradation when heated. Cannot be used to prepare samples for further analysis…
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interaxctal- shapester- organizastions-
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1.viscosity2.flash point3.effects of oxidants
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Ethanol has much better properties than water at chromatography
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it is the process in which mixtures of substances are separated into their compounds because some chemicals are heavier than others.
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In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent. Also, in some cases, DCM(dichloromethane is used as a solvent.
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thin layer chromatography uses alumna spread thinly on aluminum plates which can be made to any size that is needed,paper chromatography as the name suggests uses paper as its stationary phase and its applications are somewhat limited when compared to other forms of chromatography.the only time ive …
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vWe all know that green plants are green because they contain chlorophyll; we know that chlorophyll is green. Hang on; why are plants different shades of green if they all contain the same green pigment? Well, perhaps it is not quite as simple as you were told by your biology teacher. In fact there …
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um no one realy no but anyways you can say you did if you have a assight ment do on this
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chromatography is very important in chemistry,it is used to analyse unknown compounds to determine there structure and much other information,it is also possible to use chromatography to separate mixtures and check for side products in a syntheses.NMR spectroscopy is important in telling how many ca…
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Ion-exchange chromatography is a process that allows the separation of ions and polar moleculesbased on the charge properties of the molecules.
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physical change causes the reaction in chromatography!
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Chromatography in which separation is based mainly on differences be-tween the solubility of the sample components in the stationary phase (gaschromatography), or on differences between the solubilities of the compo-nents in the mobile and stationary phases (liquid chromatography)
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To separate or divide all the components of a mixture which consists of different substances. It can then be used to identify each and every component.
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Chromatography is an analytical method to separate the compounds from the two immiscible phases,one being a mobile phase and another being a stationary phase. It is used in petroleum industry,pharmaceutical industry.
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A meniscus is the gentle curve displayed at the top of a liquid when it is poured into a container.
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Rf is the relationship of the distance moved by a pigment to the distance moved by the solvent.
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electrophoresis: it is the separation technique based on the charge- size ratio. the molecules which are separating moves at different rate based on 1) charge 2) size 3) pore size of the gel. agarose gel is commonly used. the visibility can be increased upon the addition of fluorescent dyes with the…
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The goal of area normalization is to correct for sample size discrepances, that in a negative way affects the sum of all measured solutes. ExampleIf 4 peaks are being measured and the sum total area of all peaks turns outto be less than or greater than 100 percent, Normalization corrects for thisand…
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multiply the micromoles with molecular weight of the substance being considered.
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Total volume of mobile phase in a fully wet packed column- the space between the particles of the stationary phase plus the volume within the particles
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Complete combustion occurs when a substance is burned in excess oxygen, producing a limited number of products; incomplete combustion occurs when a substance is burned with limited oxygen, producing a more diverse range of products than that of complete combustion. When a reactant is completely com…
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In forensic chemistry!
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well the proper way to solve this is Rf=distance by traveled pigment divided by distant traveled by solvent
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scientists use chromatography because it is a tachnique that shows what is soluble and what isn't.
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what determines separation
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Predicting whether or not there is a particular substance in your given compound, such as detecting drugs in the urine.
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because it will separate the chemicals and give the investigators some evidence
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set exam eligibility crieteria for OBC
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In science, chromatography is an important technique for separating mixtures of solutes in a solvent. chromatography can be used to find base colours. the different dyes in a single colour one other use is to detect small amount of poison in liquid samples.
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