Biotechnology

Sarkosyl is a detergent commonly used in DNA isolation to disrupt cell membranes and release DNA. It helps solubilize membrane proteins and lipids, allowing for the extraction of pure DNA from the cells. By disrupting cell membranes, sarkosyl helps in the efficient extraction of DNA from various sources.

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Exons are part of both prokaryotic and eukaryotic.

Introns are rarely present in the domain bacteria (common bacteria) while introns are present in some genes in domain archaea ("ancient" bacteria). Both are considered prokaryotic. No, they are only present on tRNA and rRNA.

Liquid detergent used in the genomic DNA extraction, emulsify plasma membrane and nuclear membrane promoting lysis. SDS (Sodium Dodecyl Sulphate) is an anionic detergent used in DNA extraction. It removes the positive ions from the proteins, due to this protein loses its conformation and gets destroyed thus the cell membrane gets damaged and cell gets broken.

Yes, it is possible to pursue a PhD after completing a BPharm and LLB. However, you may need to demonstrate a strong research interest that aligns with the field of study for which you are applying for your PhD. Additionally, meeting the specific admission requirements of the PhD program you are interested in is essential.

You can check the correctness of your primer sequence by performing a basic sequence alignment using bioinformatics tools like BLAST or tools provided by your institution. Additionally, you can run a PCR with your primers and sequence the resulting PCR product to confirm that the correct DNA region has been amplified.

Transgenic bacteria are bacteria that have been genetically modified to contain genes from another organism. These genes are usually inserted to give the bacteria new functions, such as producing a specific protein or metabolizing a particular substance. Transgenic bacteria are commonly used in biotechnology and research.

Cystic fibrosis is a genetic disorder that gene therapy may correct by introducing a healthy copy of the CFTR gene into cells to restore normal function in affected individuals.

Cystic fibrosis is an example of a disease that might be treated using gene therapy. This genetic disorder affects the lungs and digestive system, and gene therapy could potentially be used to correct the underlying genetic mutation that causes the disease.

When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.

If a drug blocks the action of polymerase in a virus-infected organism, it would inhibit the virus's ability to replicate its genetic material, thus preventing the virus from making copies of itself. This would likely slow down or stop the spread of the virus within the organism, potentially helping the organism's immune system to control the infection.

Potato turns muddy green when tested with Benedict's solution due to the presence of reducing sugars like glucose and fructose. The reducing sugars react with the copper ions in the Benedict's solution to form a green-colored precipitate called cuprous oxide.

In biotechnology, vectors are used to transfer and replicate specific DNA sequences in host cells. They act as vehicles to carry foreign DNA into host cells, facilitating the production of desired proteins or genetic modifications. Vectors play a crucial role in gene cloning, gene expression, and gene therapy applications in biotechnology.

Tissue culture in biotechnology involves the growth of cells from plants or animals in a controlled environment, typically a nutrient-rich medium. This technique allows for the production of genetically identical cells or tissues, which can be used for various applications such as plant propagation, drug development, and research on cell behavior.

Biotechnology plays a crucial role in health sciences by providing advancements in areas such as drug development, personalized medicine, genetic testing, and disease diagnosis. It allows for the development of innovative treatments and therapies that can target specific diseases at a molecular level, leading to improved patient outcomes and overall healthcare.

Humans can touch most physical objects, but there are some things that are dangerous to touch, such as hot surfaces, toxic chemicals, or sharp objects. Additionally, there are objects that are too small to be touched or too far away to be reached.

A mutation is a change in the DNA sequence that can occur due to errors in DNA replication, exposure to radiation, or chemicals. Mutations can have various effects on an organism, such as causing genetic disorders or contributing to evolutionary changes.

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It depends on if you're referring to CG as an acronym or just a general reference to the genome. C, or cytosine, and G, or guanine, are two of the four bases that are the building blocks of DNA. An individual's genome, or his or her complete DNA code, is a series of these four bases. The other two are adenine (A) and thymidine (T).

Using "CG" in a different way, microarray technology is sometimes referred to as aCGH, which stands for "array comparative genomic hybridization". Microarray technology allows for the comparison of a test DNA sample against a partial control genome. This genome is represented by an array of probes, which are small pieces of DNA. How many probes one has in an array and which parts of the genome are represented customize the array to help a researcher/clinician interrogate the control sample for certain differences. They can be helpful in finding deleted or duplicated DNA material, for example.

AUFS stands for Agilent Universal Fit Sample loop. It is a type of sample loop used in high-performance liquid chromatography (HPLC) systems. AUFS sample loops are designed to be compatible with various types of HPLC systems, providing flexibility and ease of use.

Organisms get rid of waste through processes like respiration, excretion, and defecation. They take in materials they need through processes like ingestion, absorption, and assimilation. These processes help maintain the balance of nutrients and waste in the organism's body.

Western blot is an analytical method used to identify specific proteins on the sample. It is widely used in clinical labs to identify pathogens in the patient sample to conclude what disease it is. It is a powerful technique used in research labs.

Factors that affect membrane fluidity include temperature, lipid composition (such as saturated vs unsaturated fatty acids), cholesterol concentration, and presence of other molecules like proteins or carbohydrates. Higher temperatures increase membrane fluidity, while saturated fats and higher cholesterol levels tend to decrease fluidity. Proteins and other molecules within the membrane can also influence its fluidity.