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Biotechnology

Manipulating living organisms to produce a technical solution to a known problem.

3,003 Questions

Can a mpc student join in biotechnology?

Yes, an MPC student can join a biotechnology program depending on the specific requirements of the program. They may need to meet certain prerequisites or transfer to a school that offers a biotechnology program if MPC does not have one. It's important to research the program's requirements and reach out to admissions for more information.

Is there any mutation in human being?

Although a healthy human being is devoid of any harmful mutations, these can occur in humans.

In cancer for example, cells develop mutations that allow them to grow in abnormal numbers, resulting in the formation of a tumor.

To summarize, the human genome is capable of undergoing mutations in certain circumstances such as during diseases. However, the incidence of a harmful mutation in a healthy individual is quite uncommon

What is the desired outcome of gene therapy?

Gene therapy has the desired outcome of eradicating or curing diseases that are caused by a specific abnormality or mutation of a gene that is a killer. It is a developing science and slowly getting results.

How has biotechnology been used to improve potato crops?

Biotechnology has been used to improve potato crops by introducing genetic modifications for traits such as disease resistance, increased yield, and improved nutritional content. This has been achieved through techniques like genetic engineering and marker-assisted breeding, which help in developing new varieties of potatoes that are hardier, more productive, and nutritionally enhanced.

What could happen if the plasmid were cut at more than one site?

If the plasmid were cut at more than one site, it could result in the fragmenting of the plasmid into smaller pieces. This could lead to difficulties in maintaining the integrity of the plasmid during cloning processes, affecting the stability and functionality of the plasmid. Additionally, it may disrupt the insertion of foreign DNA or hinder the replication of the plasmid in host cells.

What is a polymerase commonly used for?

A polymerase is commonly used for amplifying DNA in a process called PCR (polymerase chain reaction). PCR is used to make copies of specific DNA sequences, which is essential for various genetic testing, molecular biology research, and diagnostic applications.

What is used to break apart dna fragments in a gel electrophoresis technique?

Labs typically use restriction enzymes.

'Restriction Enzymes cut DNA at specific recognition nucleotide sequences. To cut the DNA, a restriction enzyme makes two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.'

Meaning if you have a restriction enzyme that cuts at, say TA|GC, everywhere the TAGC sequence is in the DNA will be cleaved at that point. Because there are multiple instances of TAGC in the sample it leads to DNA fragments of differing sizes which then migrate at different rates under electrophoresis.

How often does DNA polymerase match bases incorrectly?

DNA polymerase has an error rate of approximately 1 in every 10^7-10^8 nucleotides incorporated. This means that it matches bases incorrectly about once in every 10 million to 100 million nucleotides during DNA replication.

How does biology affect learning?

Biology can influence learning through factors such as genetics, neural development, and brain function. Genetics can affect cognitive abilities, while neural development and brain function can impact memory, attention, and problem-solving skills. Understanding how these biological factors interact with environmental influences can help optimize learning strategies for individuals.

Why is bioinformatics important?

Same reason any statistics is important. There is a lot of data to sift through so you invent tools to sift through that data for you.

If you have a genome form some organism that is 2 million bases and you are trying to annotate the genes it would take a long time to do this manually. To avoid this you write software or code your own program that will look at the letters and find an ATG (start) and TAG (stop) and annotate that gene for you.


A bit more complex than that and there other examples but that's basically it. There is too much data to look though on your own so you make computers do it for you.

What is the use of sarkosyl in DNA isolation?

Sarkosyl is a detergent commonly used in DNA isolation to disrupt cell membranes and release DNA. It helps solubilize membrane proteins and lipids, allowing for the extraction of pure DNA from the cells. By disrupting cell membranes, sarkosyl helps in the efficient extraction of DNA from various sources.

What is the hydrogen bond between adenine and thymine and cytosine and guanine?

I don't f*cking know, how about you pay attention in school f*cking retard!

Wow someone woke up on the wrong side of the bed your grades must ha ve really sucked. You should stop being such a loser.

Do introns and exons play any role in protein synthesis?

Exons are part of both prokaryotic and eukaryotic.

Introns are rarely present in the domain bacteria (common bacteria) while introns are present in some genes in domain archaea ("ancient" bacteria). Both are considered prokaryotic. No, they are only present on tRNA and rRNA.

What is the function of liquid detergent in genomic DNA extraction?

Liquid detergent used in the genomic DNA extraction, emulsify plasma membrane and nuclear membrane promoting lysis. SDS (Sodium Dodecyl Sulphate) is an anionic detergent used in DNA extraction. It removes the positive ions from the proteins, due to this protein loses its conformation and gets destroyed thus the cell membrane gets damaged and cell gets broken.

Can you do PhD after bpharm llb?

Yes, it is possible to pursue a PhD after completing a BPharm and LLB. However, you may need to demonstrate a strong research interest that aligns with the field of study for which you are applying for your PhD. Additionally, meeting the specific admission requirements of the PhD program you are interested in is essential.

How can I check am I using correct primer sequence in my PCR?

You can check the correctness of your primer sequence by performing a basic sequence alignment using bioinformatics tools like BLAST or tools provided by your institution. Additionally, you can run a PCR with your primers and sequence the resulting PCR product to confirm that the correct DNA region has been amplified.

Why are plasmid used as vectors vectors?

They are already tailor-made to insert DNA into another organism. Scientists hijack their mechanism and replace the original DNA/RNA with their own.

Agrobacterium tumefaciens causes crown galls in some plants, by inserting its DNA into the plant's genome. To make a vector from this plasmid, researchers removed everything that was not essential for this "inserting of DNA" and replaced it with various other genes.

What are the transgenic bacteria?

Transgenic bacteria are bacteria that have been genetically modified to contain genes from another organism. These genes are usually inserted to give the bacteria new functions, such as producing a specific protein or metabolizing a particular substance. Transgenic bacteria are commonly used in biotechnology and research.

Which is a genetic disorder that gene therapy may correct?

Cystic fibrosis is a genetic disorder that gene therapy may correct by introducing a healthy copy of the CFTR gene into cells to restore normal function in affected individuals.

What is an example of a disease that might be treated using gene therapy?

Cystic fibrosis is an example of a disease that might be treated using gene therapy. This genetic disorder affects the lungs and digestive system, and gene therapy could potentially be used to correct the underlying genetic mutation that causes the disease.

What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?

When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.

What would the result be if a drug that blocks the action of polymerase was introduced into a virus-infected organism?

If a drug blocks the action of polymerase in a virus-infected organism, it would inhibit the virus's ability to replicate its genetic material, thus preventing the virus from making copies of itself. This would likely slow down or stop the spread of the virus within the organism, potentially helping the organism's immune system to control the infection.

Why does potato turn muddy green when tested with Benedict solution?

Potato turns muddy green when tested with Benedict's solution due to the presence of reducing sugars like glucose and fructose. The reducing sugars react with the copper ions in the Benedict's solution to form a green-colored precipitate called cuprous oxide.

What is the function of a vector in biotechnology?

In biotechnology, vectors are used to transfer and replicate specific DNA sequences in host cells. They act as vehicles to carry foreign DNA into host cells, facilitating the production of desired proteins or genetic modifications. Vectors play a crucial role in gene cloning, gene expression, and gene therapy applications in biotechnology.

What is tissue culture in biotechnology?

Tissue culture in biotechnology involves the growth of cells from plants or animals in a controlled environment, typically a nutrient-rich medium. This technique allows for the production of genetically identical cells or tissues, which can be used for various applications such as plant propagation, drug development, and research on cell behavior.