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Biochemistry

Biochemistry is the study of the structure, composition and chemical processes in all living organisms. It covers the structures and functions of various cellular components, including proteins, carbohydrates, nucleic acids, lipids, and other biomolecules.

8,065 Questions

Function of TEMED in sds page?

In SDS-PAGE, TEMED is used as an accelerator for the polymerization of acrylamide. It reacts with ammonium persulfate to generate free radicals, which initiate the crosslinking of acrylamide and bisacrylamide, resulting in the formation of a gel matrix. TEMED helps to ensure the proper formation of the gel for protein separation based on size.

What are the stages of the ripening process of a banana?

Stage 1 - Hard and Green

This is how bananas are when freshly picked.

Stage 2 - Green with trace of yellow

The maturing process has begun, however there is still some time before they are ready to eat.

Stage 3 - More yellow than green

The ripening has begun in earnest and the fruit is now increasing its natural sugar content, the flesh is changing from the chalky hardness of under ripe fruit.

Stage 4 - Yellow with Green hint

That familiar creamy texture is developing now, and as the natural sugar content rises; the fruit is getting ready for eating.

Stage 5 - All yellow with green tip on crown

Ready to eat! The fruit is sweet, creamy and on the firmer side. Even though it is yummy, it still does not have its complete full sweet flavor.

Stage 6 - All yellow

At the peak of freshness now, and delicious to eat. The fruit is also starting to soften as it ages and is heading towards its top sugar content.

Stage 7 - Yellow with brown "sugar spots"

The sugar content is now at its peak; the flesh is perfect and has softened for easy mashing and blending.

See Sources and related links for pictures of the different stages.

Equation for the heat of formation of carbon disulphide?

The heat of formation (∆Hf) of carbon disulfide (CS2) can be calculated using the standard enthalpies of formation of its constituent elements as follows: ∆Hf (CS2) = ∆Hf(C) + 2∆Hf(S) − ∆Hf(CS2). The standard enthalpies of formation for carbon (C) and sulfur (S) are known values and can be used in the calculation.

1.25 mg would be equivalent to how may iu of vitamin d?

There is no direct conversion between milligrams (mg) and International Units (IU) for vitamin D because they measure different aspects of the nutrient. However, a common conversion for vitamin D3 is 1 microgram (µg) = 40 IU. So, you would need to convert the 1.25 mg dosage to micrograms and then calculate the equivalent IU based on this conversion factor.

Do vitamin supplements provide the same amount of nutrients found in food?

It varies, but in most cases the answer is no. If the supplement is of a form of that specific vitamin or mineral that is derived from fresh foods, then yes. Some supplements of that type are available, but most are not. Some are forms that may be similar to what comes from fresh foods and readily absorbed by the body, even if they are not identical. In some cases, your body can utilize them the same way. In some cases, it cannot.

Vitamin C in foods comes with many co-nutrients that help the body to absorb it, deliver it to where it needs to go, and utilize it properly. Vitamin C in most supplements is ascorbic acid, which has none of the co-nutrients. Some contain bioflavonoids or some other form of a food component to aid absorption, but none are the same as getting fresh food (even the ones that are actually from food - nothing beats _fresh_ food). The same applies in other cases, though there are specific items wherein the supplement may be almost identical to the compound found in foods. It depends on the vitamin or mineral and how it is prepared. The raw, food-grown form of supplements (which is different than food-based) are the closest to fresh foods.

Answer

It depends. Vitamin C, ascorbic acid, is identical in both supplement and natural form. Others are not. (Most synthetic vitamins are not recognized by the body and are flushed out). While you may consume 100% of your RDA of a nutrient in a vitamin pill, you are probably only absorbing 10% from most pills as the binders used to compact the pills make it hard for your system to dissolve them. In the end though, supplements serve exactly their purpose- to supplement the diet and prevent deficiencies. You might eat well, but even an excellent diet won't include 100% of everything, every day. A daily multivitamin can be seen as insurance of meeting those requirements.

Fresh foods provide many more nutrients than vitamins and minerals alone, such as fiber, antioxidants and many others.

Answer

Are the vitamins and minerals in supplements identical to fresh foods? No, but they are better than trying to get everything you need out of a can.

Vitamins and mineral are great however they are not broken down and processed in the body as easily as foods are. Some vitamins such as A,D,E,K are stored in the fat cells of the body and can become dangerous if these vitamins are abused. Vitamins A,D,E,K are fat soluble vitamins. These vitamins coming from a natural food substance can be easily flushed out of the body if they become toxic, however they are harder to flush out of the body if they are taken in a pill form! All the other vitamins are water soluble which means they are stored in water and can be easily flushed out. Minerals are great for your hair,skin and nails.

Where does the carbon in the carbon dioxide you breath out come from?

The Carbond Dioxide (CO2) in expired air is the waste product of complete cellular respiration that is carries in the blood to be removed by the lungs. Each cell uses a carbon based fuel (like glucose) to get energy. The cells that contain strucutres called Mitochondria are able to cully oxidise such hydrocarbons to just CO2 and water. The oxygen comes from inhaled air, and both the hydrogen and carbon comes from these food molecules.

What is the Effect of dilution on enzyme activity?

At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.

Bio-inorganic and super molecular chemistry-structure and important reaction of vitamin B12?

Vitamin B12 is a bio-inorganic compound that contains a cobalt ion at its center. The cobalt ion plays a crucial role in the cobalamin family as a cofactor in enzymatic reactions, especially in DNA synthesis and metabolism. The structure of vitamin B12 includes a corrin ring with a cobalt atom bound to a nitrogen atom of a dimethylbenzimidazole group, a 5,6-dimethylbenzimidazole nucleotide moiety, and an α-ribazole sugar.

One of the most important reactions involving vitamin B12 is the conversion of methylmalonyl-CoA to succinyl-CoA, catalyzed by the enzyme methylmalonyl-CoA mutase. This reaction is essential for fatty acid synthesis and energy generation. Additionally, vitamin B12 plays a critical role in the one-carbon metabolism cycle, where it acts as a cofactor for methionine synthase to convert homocysteine to methionine, an important reaction in DNA synthesis and cell growth.

What is the purpose of carrying out a preliminary trial?

The purpose of carrying out a preliminary trial is to assess the feasibility and suitability of a study before conducting the full-scale research. It helps researchers identify potential challenges, refine the research methods, and determine if the study objectives can be achieved.

Formula for calculating distribution coefficient kd in size exclusion chromatography?

The distribution coefficient, Kd, in size exclusion chromatography is calculated using the equation Kd = Vt/Vo, where Vt is the total elution volume of the sample and Vo is the void volume of the column. The distribution coefficient provides information about how the sample components interact with the column matrix based on their size and shape, with larger molecules eluting faster than smaller ones.

Definition of dilution and dilution factor?

In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.

What is the principle of electrolyte analyzer?

Measurement of electrolytesElectrolytes are measured by a process known as potentiometry. This method measures the voltage that develops between the inner and outer surfaces of an ion selective electrode. The electrode (membrane) is made of a material that is selectively permeable to the ion being measured. For example, sodium electrodes are made from a special glass formula that selectively binds sodium ions. The inside of the electrode is filled with a fluid containing sodium ions, and the outside of the glass membrane is immersed in the sample. A potential difference develops across the glass membrane that is dependent upon the difference in sodium concentration (activity) on the inside and outside of the glass membrane. This potential is measured by comparing it to the potential of a reference electrode. Since the potential of the reference electrode is held constant, the difference in voltage between the two electrodes is attributed to the concentration of sodium in the sample. Ion selective membranes can be made from materials other than glass. For example, the antibiotic valinomycin is used to make potassium-measuring electrodes. Neutral carrier ionophores selective for lithium, calcium, and magnesium are also used for measurement of these substances in laboratory medicine. Ion selective electrodes can be used to measure whole blood, serum, or plasma since they respond to the electrolyte activity in the water phase of the sample only. One important aspect of electrolyte measurement is an artifact (erroneous result) called pseudohyponatremia that may occur when sodium is measured using a diluted blood sample. This happens when the plasma contains excessively high lipids or protein. These solids displace plasma water from the specimen, resulting in a low measurement of sodium that does not occur with an undiluted sample.

Total calcium and magnesium are usually measured by colorimetric procedures called dye binding assays. Calcium is displaced from protein by dilute acid or alkali and reacts with a dye (arsenazo III or cresolphthalein complexone) to form a colored product. When crosolphthalein complexone is used, 8-hydroxyquinoline is added to bind magnesium which also reacts with this dye. Magnesium is commonly measured by its reaction with a dye called Calmagite. A calcium chelator such as EGTA is added to prevent interference from calcium. Both calcium and magnesium may be measured by atomic absorption spectrophotometry. This procedure is more complex than colorimetric methods, but is also more accurate. Phosphorus is measured by reacting it with ammonium molybdate at an acid pH. The rate of ammonium phosphomolybdate formation is measured at 340 nm and is proportional to the inorganic phosphorus concentration (mono-and dihydrogen phosphate) of the sample.

Nutritional facts about apricots?

Apricots are an excellent source of vitamin A, a very good source of vitamin C, and a good source of dietary fiber and potassium. Apricots contain phytochemicals called carotenoids, compounds that give red, orange and yellow colors to fruits and vegetables. The powerful antioxidant Lycopene is one of the carotenoids found in apricots It has potassium, tryptophan, dietary fiber and many more Apricots contain phytochemicals called carotenoids, compounds that give red, orange and yellow colors to fruits and vegetables. The powerful antioxidant Lycopene is one of the carotenoids found in apricots

What are positively and negativity charged ions?

Positively charged ions, or cations, are atoms that have lost electrons, resulting in a net positive charge. Negatively charged ions, or anions, are atoms that have gained electrons, leading to a net negative charge. These charged ions are formed through the process of ionization.

What is chiral intermediate?

A chiral intermediate is a molecule that has an asymmetric carbon atom, resulting in non-superimposable mirror image structures known as enantiomers. These enantiomers exhibit distinct stereochemical properties and can have different biological activities or reactivities in chemical reactions. Chiral intermediates play a crucial role in asymmetric synthesis and the production of single enantiomer compounds.

Why do cells maintain biochemical reactions far from equilibrium conditions?

Cells maintain biochemical reactions far from equilibrium conditions to drive metabolic processes, enable energy transformation, and ensure cell survival. By constantly investing energy to maintain non-equilibrium states, cells can regulate and control reactions, allowing for efficient synthesis of essential molecules and maintenance of cellular functions.

What is the Chemical structure of NAD?

Nicotinamide adenine dinucleotide, abbreviated NAD+ is a dinucleotide, since it consists of two nucleotides joined through their phosphate groups, with one nucleotide containing an adenine base and the other containing nicotinamide.

Salary of a person who does autopsy?

The salary of a person who performs autopsies, such as a forensic pathologist or a medical examiner, can vary based on factors like location, experience, and employer. On average, they can earn anywhere from $80,000 to $200,000 per year.

Do genes provide the cell with a specific set of hormonal instructions or with a set of biochemical instructions?

Genes provide the cell with a set of biochemical instructions. They contain the blueprint for making proteins, which can include hormones. Hormones are specific types of proteins that are produced based on the instructions encoded in genes.

What did becquerel observe when he later took the items out of the drawer?

When Becquerel took the items out of the drawer, he observed that the uranium compound had fogged up the photographic plate wrapped with them, indicating that the uranium was emitting radiation capable of penetrating the paper covering the plate. This accidental discovery led to the development of the field of nuclear chemistry.

Can flip-flop of lipids in the bilayer occur without flippases?

Yes, flip-flop of lipids in the bilayer can occur without flippases, but at a much slower rate. Flippases actively catalyze the movement of specific lipids between the inner and outer leaflets of the bilayer, while passive flip-flop can happen spontaneously, driven by concentration gradients and membrane fluidity.

Examples of intracellular enzymes?

Examples of intracellular enzymes include catalase, an enzyme that breaks down hydrogen peroxide in cells, and DNA polymerase, an enzyme involved in DNA replication. Additionally, enzymes like lipase and protease function intracellularly in breaking down lipid and protein molecules, respectively.

Does essential amino acids include glycine and alanine and serine?

Yes, glycine, alanine, and serine are all nonessential amino acids, meaning our bodies can typically synthesize them on their own. Essential amino acids are the ones that must be obtained through diet because the body cannot produce them.

What is the role of 1 percent of NaOH in the isolation of RNA?

Sodium hydroxide (NaOH) is used in RNA isolation to disrupt cell membranes and denature proteins. At 1%, NaOH helps to increase pH, facilitating the release of RNA from cells and protecting it from degradation. It also helps to inactivate RNases, enzymes that can degrade RNA.