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Genetic Engineering
Questions about the manipulation of an organisms genes in order to alter the morphological or chemical traits of the organism.
1,707 Questions
Why are Plasmids and viruses used as vectors?
Transformation of bacteria plasmids are one of the easiest ways to demonstrate and test hypotheses about genetic transformations in laboratory conditions due to the ease of performing the procedure and relative quickness of obtaining results. DNA can be taken into the bacterium by subjecting the a competent cell to heat shock which makes the plasma membrane more fluid. (A bacterial cell may need to be treated with a chemical, ie) CaCl2 in E. coli, in order to become competent, or have enhanced ability for DNA uptake). The foreign DNA, or plasmid, will insert itself into the DNA of the bacterium. Bacterial cells have relatively few genes, compared to other organisms. Bacteria replicate frequently and will therefore express a phenotype in a reasonable amount of time. Only some competent cells will become transformed. This factor is expressed as transformation efficiency, which is defined by (# of transformants obtained)/(# of total cells).
Is there genetic engineering for cri du chat syndrome?
what research is currently being done on cri-du-chat
Role of mgcl2 in competent cell preparation?
The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to Lipopolysaccharide. Positively charged calcium ions attract both the negatively charged DNA backboneand the negatively charged groups in the Lipopolysaccharide inner core. The plasmid DNA can then pass into the cell upon heat shock, where cells are cooled to a low temperature (+4 degrees Celsius) and then heated to a high temperature (+42 degrees Celsius) for a short time.
Which named part in a animal cell contains genetic information?
DNA is found in the nucleus of the cell, which contains chromosomes, genes are found on chromosomes
What are the two main sources of genetic variation and which of them is more common?
The two main sources of genetic variation are sexual reproduction, and mutation. Sexual reproduction is more common.
What can cause genetic variation?
genes are kept in chromosomes, there is this thing called meiosis which is when the chromosomes/genes get split in half, one from both parents that way it equals the normal amount of genes later. the genes have tons of ways to mix and match around while getting split apart and then ending up in the same place.
An act of fertilization in which genetic information is transferred between cells is called?
One of the steps during fertilization is called conjunction. During conjunction, genetic information is transferred between cells to arm the cell with the information necessary to replicate..
How the genetic information is stored in a cell?
Gene stores information as a sequence of nucleotides, which codes for the sequence of amino acids that determine the formation of a specific polypeptide or protein.
How does a herpes virus get energy?
Like other viruses, herpes is an obligate intracellular parasite. It gets its energy from the host cell's ATP.
Why might parents decide to have genetic testing?
Parents might decide to have genetic testing to determine if either or both of them carry certain genetic traits that could be passed on to their offspring. Some genetic traits will lead to severe birth defects or problematic diseases that could severely impact the quality of their children's lives.
If they are indeed carriers of certain traits, they may choose to avoid having children or choose adoption as an alternative. In this way, they can eliminate their particular genetic line and avoid passing the trait on to the next generation.
Have there ever been any genetic experiments in medicine with positive results?
Try the recombinant ligand epoetin alfa that helps me with my anemia, or just try insulin therapy for humans. This insulin therapy means we no longer have to rely on insulin from horses, for instance, but now we avoid the harmful reactions that therapy visited on some people.
Yes, there have been genetic experiments in medicine with positive results.
Are you for or against the genetic engineering?
This is a very open ended question; some people would tell you absolutely not, and that its an abomination.
Others, such as myself, believe it is the way of the future.
Its pretty much something you'd need to do reseach into and form your own opinion. There are strong reasons to do it, but there are also risks and moral issues involved.
This is for Apex Learning The answer is
Differentiation
What is the principle of DNA isolation using phenol chloroform method?
Phenol chloroform extraction is the oldest and still widely followed method for the isolation and extraction of DNA from plant and animal cells. The phenol, chloroform (and also isoamyl alchohol) are added in a specific ratio of 25: 24:1.
Phenol: Phenol dissolves the organic impurities, like proteins etc.
chloroform: Provides density to phenol so that it settles below water during phase separation.
Isoamylalchohol: Used to prevent phosgene from reaction of chloroform with air.
The Phenol:Chloroform:Isoamylalchohol (PCI) solution is added to the cell extract after removal removal of debris. After proper mixing, cetrifugation is done to separate the phases. Two phases are formed: The upper, the aqueous phase that contains DNA, the lower phase, that phenol phase, that contains organic impurities. Thus two phases are separated by a very clearly defined boundary of coagulated proteins.
The aqueous phase is precipitated and then the DNA could be pelleted after rounds of purifications.
Does every cell have genetic material?
No, the gametes or sex cells contain only half of the chromosomes of a body cell so the gene count would not be equal.
What kind of acid is in a molecule that carries a genetic information?
Nucleic acids:
- Deoxyribose Nucleic Acid
- Ribose Nucleic Acid
Difference between an autosomal recessive disorder and an autosomal dominant disorder?
autosomal dominant is only when one allele is messed up
autosomal recessive is when you give the disease to your child 50/50 chance.
Not true.... 50/50 when its Autosomal Dominant(see below)
- Recessive Inheritance
Both parents carry a normal gene (N), and a faulty, recessive, gene (n). The parents, although carriers, are unaffected by the faulty gene. Their offspring are affected, not affected, or carriers. This type of inheritance was first shown by Mendel.
- Dominant Inheritance
One parent has a single, faulty dominant gene (D), which overpowers its normal counterpart (d), affecting that parent. When the affected parent mates with an unaffected and non-carrier mate (dd), the offspring are either affected or not affected, but they are not carriers.
How does DNA help with the transfer of genetic material from parents to offspring?
Yes, it does. You get 23 chromosomes from your mother and 23 from your father. These chromosomes contain DNA, which is the code for making genes. Since you get DNA from your parents, you also get genes from them.
How can genetic engineering reduce the use insecticides?
Genetic Engineeringcant reduce the use of insectides. In fact, there is demonstrable evidence from the US Dept of Agriculture's own data that in fact, since the introduction of Genetic Engineering there has been an overall increase in the use of insecticides.
Its perfectly logical when one considers that the companies (E.g. Monsanto) who own the patent on the Genetically Engineered (aka Genetically Modified) plant that is resistant to herbicide/insecticide, also owns the patent rights to the herbicide/insecticide. It is in the corporations best interests to increase the use of pesticides. Think about it - why would a corporation kill the goose that lays the golden egg? It pays to remember: biotechnology companies are also the world's biggest agrichemical companies.
