Microscopes

The lenses in electron microscopes are typically made from electromagnets, rather than glass lenses used in light microscopes. These electromagnets focus and direct the electron beam to generate high-resolution images by controlling the path of the electrons.

Two reasons. Firstly, charges (electrons in this case) congregate near edges (of the sample) will repel incoming electrons more so than a flat surface. Secondly, electrons hitting an edge are more likely reflected at an off angle away from the return-electron detector, weakening the intensity of the image there.

==============================

As magnification increases in a microscope, the field of view decreases, meaning you can see less of your specimen at a time. Additionally, resolution may decrease slightly, impacting the clarity and sharpness of the image. It may also become more difficult to maintain focus as magnification increases.

You can see a flower's cell and your blood celland any cell! I hope that kind of answered your question .. I'm sill studying about microscopes:)

I'm not sure if I understand the question. When using a compound microscope, you always want to start by adjusting the coarse adjustment under low power (like 4x) until you have whatever you are looking at in focus. Then, without moving the adjustment, go to the next power (like 10x or something) and use the coarse adjustment only a little and then use the fine adjustment to get it into focus again. Without touching anything, switch to the next power (say 40x) and DO NOT TOUCH THE COARSE ADJUSTMENT. You will lose your object and have to start all over. Only use the fine adjustment past this point. Again, focus as best as you can. This will depend on the microscope, but some also have a 100x power objective lens. It is best to put a drop of immersion oil onto your sample while you move from 40x to 100x. Then you should only use the fine adjustment a small amount to get your sample into focus. The immersion oil improves the resolution of the image that hits your eye, making it easier to see and not blurry.

Adjusting a microscope's magnification settings can alter an object's field of view from a macro to micro areas. Higher magnification make the field of smaller and better defined, where lower settings increases the visible area.

Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it.

It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.

You can see a hair fiber well using an optical microscope, but you can hardly see a dimension 100 times smaller (about 10 microns). An electron microscope can review features as small as 10 nm, about 1/100000 th of a fiber.

Scientists see a blur when they look at an atom under a microscope because the size of atoms is on the scale of a few tenths of a nanometer, which is smaller than the wavelength of visible light. This means that the light waves cannot resolve the details of the atom's structure, leading to a blurred image.

The scanning lens of a compound microscope is used whenever a new slide is viewed or when the view of the specimen in the field of a higher power lens is lost. Think of it as the "neutral" position for the lens array. The scanning lens has the greatest working distance of the lens group on the microscope and is far enough away from the slide to avoid crunching the slide (and possibly damaging the lens) when attempting to focus. Many microscopes are parfocal, meaning that once you have a focused view of the specimen with the scan lens the image will be in, or very near in, focus when you swivel to a higher power lens. Very important! If you can't find a good view at higher power, or you "lose" the specimen after trying to focus with the fine focus knob only, go back to the scanner lens. Never use the coarse focus with anything but the scanner lens in position. Not doing this is probably the number one reason slides get crunched. And everyone will know because it usually makes an unmistakable sound that reverberates all over the lab.

Yes, in a suspension, the particles are small enough to remain dispersed in the solvent rather than settling out. These particles can be seen through a microscope because they are larger than the molecules in the solvent.

Microscope. A scientist might use a microscope. Well actually, it depends on the size of the animal. If it is microscopic, then scientists might have to observe it using it using a microscope. If it can actually be seen, then scientists mught actually take a sample of it back to the labratory to observe physically.

If you mean magnification, unless you have a confocal microscope, the objective lens with the lowest mag will be the shortest -- largest clearance between the bottom of the lens and the stage. You can easily load and unload (slide in or out) the sample off the stage. If the reason is the above, I have a better way. I always load the sample with the lens rotated away -- the best clearance will be when no lenses are in position. If so, I would have lowered the stage quite a bit so that when I swing the lens in position, I am not scratching the sample.

However, a better reason for starting with low mag would be that you can focus much easier with the lowest-mag lens and then refocus to go to a higher mag -- the sample is approximately in focus all these times. When the sample is way out of focus, as when the highest-mag lens is on when you first insert the sample, it is hard to know which direction to turn the focus knob without scratching the sample.

The arrow would likely point to the cylinder lens, which is the objective lens of the microscope. This lens is responsible for magnifying the specimen being observed.

A simple light microscope works by passing light through a specimen, which is then magnified by a series of lenses to create an enlarged image that can be viewed by the observer. The lenses in the microscope refract the light in such a way that the image appears larger and more detailed than the actual specimen.

It moves to the left pretty much but I can,t really give you much of a scientific answer why.

Total magnification on a microscope is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece. For example, if the objective lens magnifies 10 times and the eyepiece magnifies 15 times, then the total magnification would be 10 x 15 = 150 times.

The most powerful type of microscope is the electron microscope, which uses a beam of electrons to achieve much higher magnification and resolution compared to light microscopes. This allows visualization of structures at the atomic level.

Short Answer: The unaided human eye which has a magnification of 1X and can resolve details down to about 0.2 millimeters.

Longer Answer:

Depending on the type of lens a single lens magnifier can magnify up to about 12X and resolve down to 17 micrometers.

A multiple lens achromatic magnifier can go up to about 25X and resolves down to about 8 micrometers.

Stereo microscopes that can easily view 3-D objects go up to about 1000X and resolve down to about 150 nanometers.

Likewise, any optical microscope is limited by the diffraction limit of light to about 1300X of usable magnification. More magnification will just produce a larger version of the image that is blurry since it is not possible to sharpen the focus beyond the 150 nanometer diffraction limit.

Microscopes that use ultraviolet light, which has a shorter wavelength that can focus tighter, can resolve down to 10s of nanometers or about 10 times better than visible light microscopes for a magnification of 10,000 X.

Modern Scanning Electron Microscopes (SEM) are capable of resolving down to about 0.2 nanometers which translates to a useful magnification of 1,000,000 X. Specialized types of electron microscopes (Field Emission Transmission Electron Microscopes) can resolve down to 0.05 nanometers which translates to a magnification of 2,500,000 X. For reference, a carbon atom is about 15 nanometers in diameter.

Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. It is a first step to determine the identity of a particular bacterial sample. Gram stains are performed on body fluid or biopsy when infection is suspected. It yields results much more quickly than culture, and is especially important when infection would make an important difference in the patient's treatment and prognosis.

Enlargement with a microscope is typically referred to as magnification. This term describes the process of making objects appear larger through the use of lenses and optical technology in microscopes.

A transmission electron microscopes (TEM) can magnify a sample up to one million times. The sample must be cut extremely thin. An electron beam is directed onto the sample to be magnified and some of the electrons pass through and form a magnified image of the specimen. A scanning electron microscope (SEM) can magnify a sample up to 100,000 times. A sharply focused electron beam moves over the sample to create a magnified image of the surface. Some electrons in the beam scatter off the sample and are collected and counted by an electronic device. Each scanned point on the sample corresponds to a pixel on a television monitor; the more electrons the counting device detects, the brighter the pixel on the monitor is. As the electron beam scans over the entire sample, a complete image is displayed on the monitor. SEMs are particularly useful because they can produce three-dimensional images of the surface of objects. A SEM scans the surface of the sample bit by bit while a TEM which looks at a sample all at once. The scanning transmission electron microscope (STEM)combines elements of an SEM and a TEM and can resolve single atoms in a sample.

The diaphragm in a compound microscope is an adjustable circular disk located beneath the stage. It helps control the amount of light that passes through the specimen, allowing for better contrast and clarity in the image. By adjusting the diaphragm, users can regulate the intensity and focus of the light to achieve optimal viewing conditions.