Biotechnology

I do not personally work in that field but have studied it for a long time. This can be done, it was mainly tested on goats. Their offspring were deformed or perfectly fine. Same as when they did this to try to clone pets. The turn out the same, die right away or are deformed. My answer is Yes, both when you add it after their born or to their mother before they were born.

Gene therapy is already successful for certain genetic disorders, such as SMA and beta-thalassemia, with ongoing research to expand its applications. The field is rapidly evolving, and as technology advances and more clinical trials are conducted, we can expect gene therapy to become increasingly successful in treating a wider range of diseases in the near future.

During the day - the side of the Earth facing the Sun heats up - the portion facing away from the sun is in night time - and thus is not being heated... making it cooler !

About 50 million years ago there was a freshwater arctic fern known as Azolla. It grew on the surface, but when it died it sank into the cold water, where it was covered and preserved. Over hundreds of thousands of years this fern pulled billions of tons of CO2 out of the atmosphere, causing earth to cool, and ultimately resulting in a long series of ice ages.

We could bioengineer a plant like Azolla to sequester gigatons of atmospheric CO2 for us now. But we might want something faster, and something that we could also easily shut down once we achieved the desired balance.

Science makes you to think how? why? what? about almost everything you come across in your life. When you know things better around you and want to know things which still remains a mystery, your mind is used to discover and invent ! And when you discover, invent and even capable of understanding things "Your mind is of course developing!"

it can't be heated to inactivation,instead you can just try protease K treatment

Scientists often look to insert a new gene into a vector, such as a plasmid or a viral vector. Vectors are vehicles that can deliver the gene into a host organism's cells for expression and study.

That question seems a little vague to me. However, what I can say is that stem cells are used to aid human life. They are essentially cells that have not yet developed for a specific function in the body. Because of this, they can (put very simply) be maniplated to grow into a cell with a needed function in a human. This can aid in repairing spinal injuries, and possibly eventually cure para or quadriplegic patients. It is also involved prototypically in the development of insulin-producing pancreatic cells--aiding people with diabetes in a possibly permanent manner. So, essentially, yes--stem cells can absolutely affect human life.

DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.

It is rare to publish an article for free in reputable academic journals. Most journals require authors to pay publication fees to cover costs related to peer review, editing, and production. However, some open-access journals offer free publication, but often charge article processing fees.

c-DNA library is a combination of cloned c-DNA(complementary DNA)fragments inserted into a collection of host cells which together constitute some portion of transcriptome(it is a set of all RNA molecules including m-RNA,r-RNA,t-RNA and other non-coding RNA produced in one or a population of cells) of an organism.c-DNA is produced from fully transcribed m-RNA found in the nucleus and therefore contains only the expressed genes of an organism.

Acid hydrolysis of sucrose involves using acids to break down the sugar molecule, while enzyme invertase specifically catalyzes the breakdown of sucrose into glucose and fructose. Employing acid hydrolysis would interfere with the enzyme's function by disrupting its structure or activity, ultimately thwarting the experiment’s objective of studying invertase's enzymatic action on sucrose.

The first step of a polymerase chain reaction (PCR) is denaturation, where the double-stranded DNA template is heated to separate it into two single strands. This step allows the primers to bind to the target sequence during the subsequent steps of the PCR process.

Grinding the liver helps break down the cell membranes and release the cellular contents, including the DNA. This step is necessary to access the DNA trapped inside the liver cells and to make it available for further extraction and analysis.

It would be easier for DNA ligase to reconnect two fragments cut by EcoR1, as both fragments would have compatible overhangs that can anneal together. In the case of one fragment cut by EcoR1 and one cut by HindIII, the overhangs produced by the two enzymes are incompatible, making it more challenging for DNA ligase to join them together.

yes, but there are amino acids can be represented by many codons.

To extract DNA from a person you need it in liquid form (i.e. spit) or if it's from a plant you can just grind up the plant in a blender to open up the cells. You need to add detergent and meat tenderizer. Detergent breaks down the lipid bilayer and meat tenderizer breaks down the protein surrounding the DNA. Let it sit for about 15 minutes so that everything can be broken down. Then add some isopropyl alcohol to it. The alcohol is polarized. It has a positive charge and DNA is negative by nature so it will pull the negative DNA out of everything else. You will see little bubbles or strings of white stuff forming and in a moment you will have clumps of DNA. This is as pure as you can get without being in a lab because they have stronger detergents and enzymes.

Washing cells with PBS helps to remove excess media, serum, and debris before adding trypsin. This helps to increase the efficiency of trypsin digestion and ensures that the trypsin can effectively detach the cells from the culture vessel. Additionally, washing the cells with PBS helps to maintain cell viability during the trypsinization process.

Not really " packaged " in the sense that eukaryotes package their DNA. Prokaryote DNA is diffuse throughout the cell and is in rings. Also other smaller rings, called plasmids, are also part of prokaryote genetic complements.

DNA ligase joins the Okazaki fragments together on the lagging strand during DNA replication. It catalyzes the formation of phosphodiester bonds between the fragments to create a continuous strand.

Must use the forward and reverse primers to bind to complementary sequence at the 3' end of the template strand - each NEW strand is built in 5' to 3' direction.

They flank the targeted gene region - must attach one to each strand of the target DNA.

A concentration of 70% alcohol is generally preferred for sterilization during plant tissue culture. This is because a 70% solution is more effective at penetrating the cell walls of microorganisms, making it a better option for disinfection. Using absolute alcohol may be too harsh and could damage plant tissues.

It is difficult to determine without more specific details about the experiment. Common issues could include a lack of control group, insufficient sample size, bias in data collection, or flawed methodology. It is important to identify and address any potential flaws to ensure the experiment's validity and reliability.

Folded membrane extending out from the nucleus is known as the endoplasmic reticulum. It plays a critical role in protein and lipid synthesis, as well as the transportation of molecules within the cell. There are two types of endoplasmic reticulum: rough endoplasmic reticulum (with ribosomes attached) and smooth endoplasmic reticulum (without ribosomes).