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Biochemistry
Biochemistry is the study of the structure, composition and chemical processes in all living organisms. It covers the structures and functions of various cellular components, including proteins, carbohydrates, nucleic acids, lipids, and other biomolecules.
8,065 Questions
isoenzyme is each of two or more enzymes with identical function but different structure.
Hope this Helps.
Generally speaking, the requirement to "start" the Calvin cycle is darkness. Remember that the first step of the first stage (or production phase, where three molecules of Ribulose-5-phosphate, or Ru5P, react with three molecules of CO2 to yield six molecules of glyceraldehyde-3-phosphate, or GAP, at the expense of nine ATP and six NADPH molecules) is the phosphorylation of Ru5P by a phosphoribulokinase to form ribulose1,5-bis-phosphate (RuBP).
The main purpose of plants' Calvin Cycle, occurring in chloroplasts, is to incorporate CO2 into carbohydrates, mainly to form glucose, by means of the ATP and the NADPH from the light reaction of photosynthesis.
What are abnormal results in a Bence Jones protein test?
Bence Jones proteins are present in 50-80% of people with multiple myeloma.Certain nonmalignant diseases, such as rheumatoid arthritis.High doses of penicillin or aspirin before collecting the urine can give a false positive result.
Rabbits are kind of animals.No animal have chloroplasts
What are made up of amino acids?
Proteins are made up of amino acids. Amino acids are the building blocks of proteins and are linked together in specific sequences to form unique protein structures that perform various functions in the body.
We can consider a protein as a product because comes from a "process" of production that in biochemistry is known as "protein synthesis" or, in molecular biology, as "translation".
Is salt a chemical or physical property?
Salt is a chemical compound. It has chemical and physical properties.
Can the melting point of a substance change over time?
does the melting point of a substance change over time
Is it okay to leave a hot plate that is in use?
never, ever!!! it is very dangerous to leave a hot plate or even a cooking stove unattended
!!
Should the police get your DNA sample?
In most of the countries the police are not allowed to get your DNA sample without the permission of the court.
Does glycine contain peptide bond?
No, glycine is the simplest amino acid and does not have any peptide bond.
What molecule does oxygen became a part of at the and of the electron transport chain?
The oxygen combines with two hydrogens and the requisite electrons to become water.
Calculate the energy per photon of radio waves of frequency 107.3 MHz?
E = hf
The energy per photon is equal to Planck's constant times the frequency, in this case 6.62606957×10−34 x 107.3x106
According to the US Environmental Protection Agency, "agricultural waste" is made up of those materials such as manure and animal output, in either solid or liquid form, from poultry and other livestock operations. It also includes harvest remains from grain, oilseed, vegetable, and orchard crops.
They are all a potential resource, since they have multiple uses, both on and off the farm.
How many years does it take to decompose foil?
If you are talking about aluminum foil, it takes a very long time. Aluminum is already in its elemental form, so there is no 'compound' to break down. Aluminum does not rust or corrode under normal exposure to the environment. The small amount that oxides on on its surface act as an inhibitor to further oxidation. So, an aluminum foil that is buried in a dump would last longer than our lifetimes.
What does a a DNA molecule consist of?
DNA molecule consists of the two chains, made up of phosphate and sugar molecules, alternately. The two chains are placed parallel to each other and are so called anti parallel to each other, because of their reverse direction to each other. Each sugar is attached to one of the base. There are four bases in DNA molecule. They are adenine, guanine, cytosine and thymine. Here adenine always pairs with thymine and guanine always pair with cytosine. The two strands are coiled like a springs around each other. Two bases are attached to each other with hydrogen bonds.
What atom is in every living thing?
do you suppose why are atoms important for living things?
...because atoms make up the matter and mater are in living things...
without atoms, proteins and others won't be made too
Does the element fluorine have anything unique about it?
Fluorine is unique because it is the most electronegative element, meaning it has a strong ability to attract electrons. It is highly reactive and can form compounds with almost all other elements in the periodic table. Fluorine is used in various applications, such as in toothpaste to prevent tooth decay and in refrigerants and plastics production.
What is the purpose of the NaNO2 when preparing the Pauly reagent?
In the presence of sodium nitrite and hydrochloric acid, sulfanilc acid form a diazonium salt. This salt react with and is an indicator for thyrosine, histidine.
What are the standard chemicals used in lab for protein estimation?
There are several methods for protein estimation, these are the most common:
- Near UV Absorbance (280 nm). Quantitation of the amount of protein in a solution using a simple spectrophotometer. UV detection focuses on Tyr and Trp content, and to a very small extent on the amount of Phe and disulfide bonds.
- Far UV Absorbance (205 nm). Detects various side chains, including those of Trp, Phe, Tyr, His, Cys, Met, and Arg.
These two methods uses: 0.1 M K2SO4 (pH 7.0), 5 mM KPO4 buffer (pH 7.0), Non -ionic detergent (e.g., 0.01% Brijn 35), and Guanidinium-HCl.
- The Lowry Method. This method is based on both the Biuret reaction, in wich the peptide bonds of proteins react with cooper under alkaline conditions to produce Cu+, which reacts with the Folin reagent, and the Folin-Ciocalteau reaction, where phosphomolybdotungstate is reduced to heteropolymolybdenum blue by the cooper-catalyzed oxidation of aromatic amino acids.
This methods requires: Complex-forming reagent (composed by the Solution A: 2% (w/v) Na2CO3 in dH2O; Solution B: 1% (w/v) CuSO4 5H2O in dH2O; Solution C: 2% (w/v) sodium potassium tartrate in dH2O); 2 N NaOH; Folin Reagent (commercially available); and protein standards (bovine serum albumin fraction V is widely used).
- The Bicinchoninic Acid (BCA) Assay. This method is similar to Lowry method, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions. The Cu+ is then detected by reaction with BCA.
This methods requires: Reagent A (0.1 g sodium bicinchoninate, 2.0 g Na2CO3 H2O, 0.16 g sodium tartrate dihydrate, 0.4 g NaOH, 0.95 g NaHCO3, made up to 100 mL. Adjust pH to 11.25 with NaHCO3 or NaOH, if necessary; Reagent B (0.4 g CuSo4 5H2O in 10 mL of water). The Standard Working Reagent (SWR) is made by mixing 100 vol of A + 2 vol of B.
- The Bradford Assay. This method relies on the binding of the dye Coomassie Blue G250 to protein.
This assays requires a solution of 100 mg Coomassie Blue G250 in 50 mL of 95% ethanol, mixed with 100 mL of 85% phosphoric acid and completed to 1 L with distilled water.
These are the most widely used assays for protein estimation.
Why do promoters need to be transferred along with desired genes in gene technology?
to ensure the genes are identical
