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Biochemistry

Biochemistry is the study of the structure, composition and chemical processes in all living organisms. It covers the structures and functions of various cellular components, including proteins, carbohydrates, nucleic acids, lipids, and other biomolecules.

8,065 Questions

How do you name peptide bond according to IUPAC?

Peptide bonds are named using IUPAC nomenclature based on the amino acids involved in the bond. The name is derived by combining the names of the two amino acids, with the N-terminal amino acid listed first followed by an arrow (-->), then the C-terminal amino acid. For example, a peptide bond between alanine and glycine would be named "alanylglycine."

Explain the relation between vitamin b12 and pernicious anemia?

first of all its called vitamin b12 deficiency anemia. Pernicious anemia and vitamin B12 deficiency anemia are the same thing. Pernicious anemia is caused by a lack of vitamin B12. You're not able to absorb the vitamin B12 due to the absence of intrinsinc factor.

Why does protein have an absorbance peak at 280nm?

When a protein in solution is analyzed using UV-visible, a peak at 280 nm is commonly observed. This peak is due to the effect of aromatic rings in the polypeptide chain (from amino acids tryptophan and tyrosine).

Does active transport require transport proteins?

Yes, active transport does require transport proteins. These proteins use energy to move molecules against their concentration gradient, typically from an area of low concentration to high concentration. This process is essential for maintaining cellular homeostasis.

Some viva voce questions for electrophoresis?

1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANT

APPLICATIONS OF ELECTROPHORESIS?

Ans. Movement of charged particle in the electric field either towards cathode or anode when

subjected to an electric current is called electrophoresis.

The following factors influence the movement of particles during the electrophoresis.

(a) Electric current.

(b) Net charge of the particle.

(c) Size and shape of the particle.

(d) Type of supporting media.

(e) Buffer solution.

Important Applications of Electrophoresis

The technique of electrophoresis is used to separate and identify the

(i) Serum proteins

(ii) Serum lipoproteins

(iii) Blood hemoglobins

2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?

Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937

(b) Zone electrophoresis: In this type of electrophoresis different types of supporting media

are used. These are;

(a) Paper electrophoresis

(i) Whatman filter paper(ii) Cellulose acetate

(b) Gel electrophoresis

(i) Agarose.

(ii) Polyacrylamide gel (used for the separation of isoenzymes).

(iii) SDS-PAGE.

(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).

(v) Immuno electrophoresis (for the separation of immunoglobulins).

What is a genosperm?

There is no specific term "genosperm" in biology. It may be a typo or a mistake. The correct term is gymnosperm, which refers to a group of seed-producing plants with naked seeds, like conifers and cycads.

What molecule is the final electron acceptor at the end of the cellular respiration transport chain?

The final electron acceptor at the end of the cellular respiration electron transport chain is oxygen. Oxygen accepts electrons and protons to form water during the process of oxidative phosphorylation.

Explain why a molecule formed by two identical atoms is nonpolar?

This is only true if "diatomic" refers to 2 identical atoms (i.e., same element)

Since 2 atoms form a straight line, the compuond is automatically symmetrical. Also, since the atoms are identical, the pull on electrons is equal, and the electrons are shared evenly. Therefore, since the charges are equal all across the molecule, no poles form. It is nonpolar.

What kind of dirt gets onto pennies?

Dirt that gets onto pennies can come from various sources such as handling the coins with dirty hands, exposure to elements like moisture or humidity, or being in contact with other dirty objects or surfaces. Over time, the dirt can build up on the surface of the pennies, giving them a grimy or tarnished appearance.

Which process represents a chemical change...Melting of ice OR Evaporation of water?

The process that represents a chemical change is the evaporation of water. This is because the bonds between the H and O atoms in water molecules are broken during evaporation, leading to a change in the substance's chemical composition. The melting of ice, on the other hand, is a physical change where the state of water changes from solid to liquid without altering its chemical structure.

Enzymes that cause hydrogen peroxide to decompose are called?

catalase enzymes. Catalase enzymes help to break down hydrogen peroxide into water and oxygen molecules, preventing the accumulation of toxic levels of hydrogen peroxide in cells.

How does paramecium expel water?

Paramecium expels excess water by contracting its contractile vacuole, a specialized structure that accumulates and pumps out water from the cell. This helps maintain the proper internal environment and osmotic balance within the cell.

Is kids allowed to drink vitamin water?

It is generally safe for children to drink vitamin water in moderation as part of a balanced diet. However, it is important to check the label for sugar content and artificial additives, and consult with a pediatrician if you have concerns about your child's nutrition.

What are glucose test results?

Glucose test results indicate the amount of sugar (glucose) in the blood. Normal fasting glucose levels are typically between 70-100 mg/dL. Elevated levels may indicate diabetes or pre-diabetes, while low levels may indicate hypoglycemia.

What does vitamin D do for the human body?

Vitamin D helps regulate calcium and phosphorus levels in the body, which are important for maintaining healthy bones and teeth. It also supports immune function, muscle strength, and cellular growth. Additionally, vitamin D plays a role in reducing inflammation and may help protect against certain diseases like osteoporosis and certain types of cancer.

What are metabolic enzymes?

Metabolic enzymes are acidic proteins that exist in the mitochondria in every cell. Their function is to process ingested proteins and sugars into energy which your body will use to function or replicate cells.

Thin filaments of water molds?

Thin filaments in water molds, known as hyphae, are essential structures for nutrient absorption and growth. They form a network called mycelium, which helps the water mold to invade and digest its food source. These filaments are typically transparent, enabling water molds to colonize various aquatic environments.

Does low density lipoproteins carry cholesterol?

Yes, low-density lipoproteins (LDL) are known as "bad" cholesterol because they carry cholesterol from the liver to cells in the body. High levels of LDL can lead to cholesterol buildup in blood vessels, increasing the risk of heart disease.

What happens to each of the functional groups on an amino acid when the amino acid becomes an ion?

When an amino acid becomes an ion (by gaining or losing a proton), the amino group (NH2) becomes NH3+, the carboxyl group (COOH) becomes COO-, and any side chain functional groups may be affected depending on their specific properties. The net charge of the amino acid will depend on the balance of protons gained or lost.

Can you take calcium and ergocalciferol with vitamin b complex?

I was just prescribed Ergocalciferol by my neurologist and his advice to me, after reviewing my RX and vitamin regime was: Take straight Calcium tabs at 2 500 tabs daily, not the Calcium augmented with vit D, lay off the B-complexes, keep up my glutcosamine and fish oil, and my white and honeybush teas were OK.

Although its usual purpose is to boost severe calcium and/or Vitamin D deficiency, it can be used for secondary uses. The reason why you are taking Ergocalciferol is the pivotal point in your question, and only your doctor can wisely answer you.

Hope this helps.

~ Its not the years, its the mileage ~

What explains the attraction of water molecules to one another?

Attractions between water molecules are called Hydrogen bonds.

The higher melting and boiling points of water suggest that water molecules attract each other more that dihydrogen sulfide molecules do. Water molecules attract each other, but these attractions are not as strong as the bonds holding oxygen and hydrogen atoms together withing a molecule.

Which liquid prevents rusting?

One common liquid that prevents rusting is oil. It creates a protective barrier on the metal surface, preventing exposure to moisture and oxygen, which are the primary factors that cause rusting. Other alternatives include grease, specialized rust inhibitors, and certain coatings such as paint or varnish.

What happens to an enzyme when it is destroyed?

When an enzyme is destroyed, its structure is altered by factors such as high temperature or extreme pH, resulting in loss of its catalytic activity. Once destroyed, an enzyme cannot perform its biological function, leading to impaired biochemical reactions in the cell or organism. The destroyed enzyme is typically broken down into its component amino acids by proteolytic enzymes in the body.

Why is vitamin D good for your skin?

Fundamentally vitamin D is used in the body for healthy bone development but like most vitamins it plays a part in multiple biochemical processes that our bodies depend upon.

Vitamin D can help with skin conditions like psoriasis because it is known to have a regulatory effect on the peptide responsible for it called cathelicidin.